Biology assay
All bioassays were performed on representative test organisms reared in the laboratory. The bioassay was repeated at 25 ± 1 oC according to statistical requirements. Assessments were made on a dead/alive basis, and mortality rates were corrected using Abbott’s formula. Evaluations are based on a percentage scale of 0-100 in which 0 = no activity and 100 = total kill.
The acaricidal activities of the title compounds were tested against Spider Mites (Tetranychus cinnabarinus). The leaves of kidney bean plants were punched into 3cm-diamter discs, using leaf punch, and put onto moist filter paper in a 7 cm-diameter styrol cup. Ten larvae spider mites (Tetranychus cinnabarinus) were put into each leaf. A 5% emulsion of a compound was diluted with water containing a spreading agent to give a 1-10 ppm solution of the compound. The solution was sprayed over each cup in an amount of 2 mL/cup, using a rotary sprinkler. After 96 h passed, the mites in each cup were observed, and mortality of the mites was dertermined. The results of acaricidal activity are listed in Table 1.
Acknowledgements
We are grateful to the National Key Project of Scientific and Technology of China (NO. 2011BAE06B01-07) and National Program on Key Basic Research Project (973 Program No. 2010CB735601) for financial support.
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