Elucidation of a new oleanane glycoside from Barringtonia asiatica

Figure 3 Sugar analysis. A sample of ranuncoside VIII (0.5 mg) was hydrolyzed in 3M HCl (0.5 mL) at 100 oC for 4 hr. The sample was taken to dryness, and the trimethylsilyl sugar derivatives were prepared for GC analysis by adding 50 µL dimethylformamide and 50 µL bis(trimethylsilyl) trifluoroacetamide containing 1% chlorotrimethylsilane to the dry powder, followed by heating at 70 oC for 15 min. Acknowledgments We are grateful to Brigham Young University for financial support and to Du Li and Bruce Jackson for help with the NMR and the MS experiments, respectively. References 1. Pratt, G. Pratt’s Grammar and Dictionary of the Samoan Language; Malua Printing: Apia, Western Samoa, 1862. 2. Cox, P. A. Economic Botany 1979, 33(4), 397. 3. Andrei, C. C.; Viera, C. P.; Fernandes, J. B. Phytochem. 1997, 46, 1081. 4. Lambert, N.; Trouslot, M. F.; Nef-Campa, C. Phytochem. 1993, 34, 1515.