The Philippine journal of science. [Vol. 57, no. 1]

-s -is *s * * - s o ry

430 The Philippine Journal of Science 1935
of virulent blood a fortnight later produced a slight temperature reaction
in the first animal and clinical rinderpest in the other, but the animal
completely recovered.
Vaccine 11. —Fuga cattle 3417 and 3402 each received the standard dose
of vaccine three days and one month old, respectively. The virulent blood
injection gave no reaction to the first, and rinderpest symptoms in the
other were followed by uneventful recovery.
Vaccine 12.-Fuga cattle 3423 and 3401 both were inoculated with the
standard dose of the vaccine four days and one month, respectively, after
its preparation. The test virus elicited no reaction in the first animal,
while the second animal showed a temperature reaction. Both animals
were completely protected.
Vaccine 13.-Fuga cattle 3261 and 3409 each received the standard dose
of the vaccine three days and one month old, respectively. No reaction
resulted after the test dose of the virulent blood, showing that a solid immunity was conferred upon both animals.
Vaccine 14.-Fuga cattle 3407 and 3411 were injected with 10 cc of the
vaccine three days and one month old, respectively. At the end of fourteen
days they were given the standard dose of virulent blood subcutaneously.
The first animal presented no symptoms whatever, and the other responded
by having a thermal reaction only.
The accompanying table summarizes in detail the tests made
on the fourteen lots of vaccine just described:
TABLE 1.-The tests of fourteen lots of vaccine and the results.'

Animal Vaccine Concen- Age of Date D Virus Date
No. No. tration. vaccine. injected. ose.. injected. Result..b njected
1I 1~ I -1

Mos. days 1934 cc. cc. 1934
3101 1 2:8 0 7 Mar. 31 10 1 Apr. 13 No reaction.
86 1 2:8 1 0 Apr. 23 10 1 May 9 Do.
3256 1 2:8 3 0 June 29 10 1 July 13 Temperature reaction.
108 2 2:8 0 9 May 7 10 1 May 23 No reaction.
3274 2 2:8 2 0 July 7 10 1 July 20 Do.
3242 3 3:8 0 3 May 28 10 1 June 13 Temperature reaction.
3272 3 3:8 1 0 June 30 10 1 July 13 Do.
3247 4 3:8 0 3 June 12 10 1 June 27 Do.
3414 4 3:8 2 0 Aug. 9 10 1 Aug. 22 Killed for vaccine.
360 5 3:8 0 3 June 29 10 1 July 13 Temperature reaction.
3412 5 3:8 2 0 Aug. 16 10 1 Aug. 29 Killed for vaccine.
a Controls: Each vaccine test was accompanied by one or two controls, which developed
typical rinderpest, and were killed for vaccine six days after virus inoculation without
exception.