Phytoplankton of the seasonal surveys of 1977, and further pre- vs. post-operational comparisons at Cook Nuclear Plant / John C. Ayers, Susan J. Wiley.

phytoplankton station collections, and master lists of phytoplankton collected,
which were not in the company report have been added.
TECHNIQUES
Phytoplankton samples are collected by Niskin bottle from a depth of
1 m, with the exception of the nearshore stations. Nearshore collections
(serial number zero stations) are made by submerging an open 1-liter bottle 4
inches below the water surface. All samples are 1-liter whole samples. Each
sample is fixed with Utermohl's iodine fixative immediately after collection
and stored in an opaque container.
In the laboratory, each sample is concentrated to 100 ml by settling
in a 1000-ml graduate cylinder and siphoning off 900 ml of fluid. The
concentrated sample-is stored in a 100-ml opaque bottle.
The samples of 1971 and of April 1972 were prepared and counted by the
Utermohl technique: placing an aliquot of the concentrated sample in a
tubular combination settling and counting chamber and allowing the aliquot to
settle overnight. The counting chamber containing the settled cells was then
separated from the settling chamber, covered, and placed on the microscope.
The samples were counted on a binocular inverted microscope at 100OX
magnification.
Beginning with July 1972, and continuing since, the method of
concentration for species identification and enumeration has been the
settle-freeze method as proposed by Sanford et al. (1969). The method
entails two days' settling of 1000 ml of sample in a graduated cylinder. The
third day the top 900 ml are siphoned off and discarded. Part of the
remaining 100 ml is used for preparation for the microscope slide and the
rest is kept for any possible further references or back checking.

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