The phytoplankton of the Cook Plant monthly minimal surveys during the preoperational years 1972, 1973, and 1974 / by John C. Ayers.

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7 -7 30
4- 20
3 --- NDGC-7-1.SnC^5 -I< —I -.-^ -.5-1 —10
0o sDc-7-l ------.^o^^R ocRgo --- ~-2-O NDC-7-I
0- I I I I o I S IHORE
7 4 2 1.5 0.51 2 4 7 MILES
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FIG. 1. The Cook Plant minimal survey grid used in months between seasonal
surveys. The reference stations, SDC-7-1 and NDC-7-1, were not used in
1972 or 1973. Contours off shore are depths in meters.
using the Settle-Freeze method of Sanford, Sands, and Goldman (1969). After
preliminary settling of the one-liter samples, 900 ml of the supernatant
liquid was siphoned off, and the remaining 100 ml swirled to resuspend the
settled material. Of the latter, 18 ml were placed in a settling chamber
mechanically held to a microscope slide and settled for two days. The
chamber was then very gently moved to set upon a block of dry ice until the
bottom 2 or 3 ml of the liquid column were frozen. The supernatant was decanted. The chamber was then removed from the dry ice and allowed to thaw
until it could be removed —leaving a wafer of ice and water on the slide.
Dehydration was accomplished by placing the slide in a chamber of anhydrous
alcohol vapor followed by a toluene vapor chamber. Cover glasses were
attached with Permount.
In examination, a horizontal and a vertical row across the slide were
counted and identified at 1000X under oil immersion on a Leitz Ortholux micro
scope. Counting methodology was the same as with the Utermohl technique.

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