Summary Statement: ADARC Program Project on HIV/SIV Vaccine Development

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1 P01 AI43042-01 23 SEP
Ho, David D.
The standard assays for monitoring the immune responses elicited by the
proposed vaccines will be carried out according to published procedures.
Characterization of humoral and cellular responses will be performed and
parameters, such as the measurement of cytokine production, will be
quantitated. Molecular analyses, such as TCR repertoire analysis and MHC
typing, will be available.
The laboratories are well equipped to conduct the proposed assays and the
personnel is qualified to provide the Core services without any serious
limitations.
The requestedtbudget for the Immunology Core is appropriate.
REVIEWER C: This core is able to meet its objectives of quan-titation of
specific humoral responses (neutralization assays, antibodyfinding, and
titration), lymphocyte proliferative responses, CTL responses, TCR repertoire
analysis, MHC typing, ADCC, CD8+ suppressor activity, chemokine assays,
macrophage and dendritic cell generation and characterization, and
immunochemical staining. As written, this core is critical to the objectives
of this P01 application.
Human Subjects: code 10
Women and Minorities Representation: not applicable
Animal Welfare: code 30
Budget: The proposed budget is recommended or approval as requested.
IRG Action: This core was rated a appropriate.
CORE C: Primate Core (Dr. P. Marx)
Critique: The comments below represent essentially unedited comments from the
reviewers of this application. They are included to indicate the range of
comments made during the discussion, and may not reflect the final thinking of
the committee. The RESUME and OVERALL CRITIQUE sections above summarize the
final opinion of the committee after the discussion.
REVIEWER A: The Primate Core will provide in-depth support for all three
projects of this application involving SIV/SHIV studies in macaques. The
staff of the core is extremely experienced in providing support and resources
for infectious disease research in primates, and is entirely appropriate for
conducting this work.
The Aaron Diamond AIDS Research Center has a long-standing relationship with
the Laboratory for Experimental Medicine and Surgery in Primates (LEMSIP), and
has maintained a laboratory and offices there for a number of years. All

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