Department of Health and Human Services Public Health Service, Grant Application

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Principal Investigator/Program Director (Last, first, middle)

Duesberg, Peter H.

Benefit Rates 1994 1995 1996
Summer Salary (Duesberg) 9.50% 9.50% 9.50%
Academic staff (Li) 21.00% 22.00% 23.00%
Students (Ellison) 1.74% 1.74% 1.74%
GSHIP 3.12% 3.12% 3.12%
Partial Fee Remission 9.23% 9.23% 9.23%
Equipment
Between equipment within the laboratory and shared facilities within the building, we
are well equipped for all of the types of experiments outlined in the grant. We
anticipate no additional purchases of equipment during the project period.
Supplies and Other Expense
At Davis, a total of 560 male, adult, CD-1 mice will be used in this study. In the first
year, 140 will be exposed to the amyl nitrite vapor at 330 ppm for six months, as
described by Al-Bayati and Raabe, 1992, and 140 will be used as a control. In the second
year, 140 mice will be exposed to amyl nitrite and clean air as described above, but at a
lower or higher concentration or for a longer exposure time depending upon the result
of the first exposure. The animals will be observed daily and clinical signs will be
recorded and evaluated. Animals will be weighed twice a month. Five to ten exposed
and five to ten control animals will be sacrificed after one to two weeks. Thymus,
lymph nodes, spleen, liver, kidney, lung and brain will be taken for gross and
microscopical examination as described by Al-Bayati, et. al, 1989. Blood and serum
samples will be taken from each animal for standard hematology and serum chemistry
analysis as described by Al-Bayati, et. al, 1991.
Funds requested in Year One for non-animal costs are based on actual historical
consumption of these items. Funds for animals and animal care are based on rates at
UC Davis. Figures for years two through three reflect a 4% adjustment for anticipated
price increases.
Travel
Funds are requested for two round trips per month between UC Berkeley and UC
Davis, to be undertaken by the Berkeley research personnel, at 136 miles per round trip,
reimbursable at $.24 per mile. These trips will be for the purpose of co-examination of
the mice exposed to nitrite inhalants, and participation in evaluation of the progress of
the toxicity studies.

PHS 398 (Rev.9/91) (Form Page 3) Page 9 EE
Number pages consecutively at the bottom thoroughout the application. Do not use suffixes such as Sa, 3b.