Bridging the Gap: Conference Workbook [International Conference on AIDS (12th: 1994: Geneva, Switzerland)]

Sequence-based HIV genotyping allows early assessment of drug resistant
mutations and may provide a guide to antiretroviral treatment.

5

VIROLOGICAL STATUS
Failure
detectable LevelA-- - - - oln e e o nse
- Baseline -+ Antiretroviral Treatment
TIME

HIVentyprk
WenGenKit

Visible Genetics' TruGene'"
HIV-1 Assay contains
everything required to
genotype HIV samples
including reagents, protocols,
and software to sequence the
3' and 5' Protease and Reverse
Transcriptase regions. HIV
genotyping services, quality
control and training are
available from our reference
labs in North America
(Applied Sciences Inc.) and
Europe (ACTGene S.A.).
700 Bay St., #1000
Toronto, ON Canada MSG 1Z6
www.visgen.com
[email protected]
N. America: Tel. [phone]
Fax [phone]
Toll Free.: 1-888-463-6844
Europe: Tel. [phone]
Fax [phone]
*The PCR process is covered by
patents owned by Roche Molecular
Systems, Inc. and F. Hoffmann-La
Roche Ltd.
For research purposes only. Not for
use in diagnostic procedures.

MicroGene Clippers"M

TruGeneTm" HIV-1 Assay

TruGeneiM Report
PROTEASE GENE
GENOTYPIC INDENTIFICATION OF ANTI RETROVIRAL RESISTANCE
Subs44tutio increased Anti-Retro Virog- CASE OVA 00
(HXB2R -, Patient) Resistance Sensitwvity P,.te..e Inhibitor S... Jtvity(S, R, Lev ofR) S OT,uREP h J, 15i99
V3SMPLEESOURCE P A30"
T12K 0 18H S37N RITONAIVIR R DAIDITE DRAIN U 2103 1997
K20I II Ir SAOIJINAVIR R DATE RECEIVED (204,1997
M461 10 Rit fNei NEI FINAVIR R VIRAL LOAiD;0 212 -p.-,ý,l
L6P172S Saq
18V Ind-Sac Ri Nel
ýA71V Ird Rit N.
L9M nd RI Saq Nei
Protease Inhibitor, I Referen-ss
7S 400, 8-0eý It 1 AIDS 1996 10 S 15 S19
0IIC C-,, II al Natý,,, 1995 374 569 71
4. 50 Candra It at J Vr., 1(996 7U 8270 76
200
i;;Ebed, tr at Aid, Re, I.,) RIIP, I ýýl 11 171 71
4,k 001Ui J-b-.1.1 AR n 19W 29. 95 97
J Ibsn t 11 J I-f Dlý 199e 113 1379-87
Me,,~ It at IAIVN 1995 3 8-13
Drug NAME: 1,6616Bi 6l8196 lt 01197 10104197 104 ý19 106 37197 107 )8/97 019 02: Coo ment J,I19562482
PaýII ts It.1 AIDS 1995 69 S27 S32
TTsdals It A AAiC 1995. 39 39 1704 10
Arrun. Asicl Mulationn. Legend
-ull,..,..........,.,,,
elm 5 I C.~~....~.....
NELFINAV R 1 0 a 0 0 a 9 a

Easy-to-use, high-throughput automated DNA sequencing system and
clinical genotyping software.
High accuracy sequencing analysis with auto-resolution detection library
and report generation.
Protease and RT regions (3' & 5') amplified by RT-PCR* and sequenced
by CLIPM.