Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Mo.A. 1036 - Mo.A.1042

Monday, July 8, 1996

air on cf is dN6 a ther r al hr patter n oranly restricted to hulI length niRNA roil
psirti cs (re5not uobcirsod.fire clltypo spec iiity of expression scorns to resideinth
HERV-K iT.tle civtty in dircel is' esranrd therirmoduirtion by a runcr" f 55 i
ronssativats ro beenstodirdsn rts iect tsinlectric iassys usng thse ii f'rs sogrio
as report0errgeti.
Results: Some, lbt rot allHLPV K LT ispa rcih 3activty in teritocaisco re cel line s
ws'hie this'arrivity is s hop itenir ell inc(hioph.2)ither-ilimted, if detestablIe it ill Ins sots irfIctiror o.iy'.it nresoeotho ictiily of the HIlV LIPR irstecatos iccinomcolls by the
factor 3I0. Ibut doesnrit is chagetIe civsty of the HERV-K Lt P. Sim.iarresults hive berec
obitaire on r h the trais activa rsi 15oein1)01 of the hosean foamy virus. In conrstt HBx (a
HeiisiB vriiprin)andsFhis(airai siissto ofidecovirnses.ncrease the HERV-K
IT aPctivity by aifactos 0
Conclusions:'[thi gh HERV K prov 5 sec ire presert in evoery uron ncell, ticrc expressioc
Iissiitsed Thre ictivity of thcii l.Td icn be ccnhraccod bycsome cil ti rins-actvatsrswhich
iso brcicved tor Ict inrrrctl/y yscheictivaio of collularitracriiption hictors Irks NFI"B and
At-2. Trais-a5 ctivaIors is awhicl inir Ito spechifctrgot sequrercc, do not rctrvate the
It Ps of the Irmin isociogouscreroviru1riamily IALPV-K.
P. Lowoc, Parill hr Inch Irs hr at, PasrI [hr Iich Sti. 5Ir-9, D-61225 [scigen. Vlephione' ~+2k6103 -77340c0 Fhis 4103-71r r2a5rossai lcacwessamus ni r nkfvct.dc
Mo.A. 1036
ANALYSIS OF IN VIVO GENETIC VARIABILITY OF VIF FROM HIV-2
rhbe roAf 1,Satst lessrreisM..,M niz PeriaijcBirhora, 1' Micro biology
Dept.PhrrssacyIcusorry cirbon, 1600 Iisboisisn Ins t Suap.Cikni. di Sivdo, 2825
Mocre di h ap riis, Portugal.
Objective: 3Ta ci r 5 ter icc Vif asrtein encoded by HIV typae 2 fromosruesra AIDS pirerits.
Idestiiaion cf[Hal/2 p's.i ots fc in rer todefse pvtatire fhississn ialraoihaposbly
icvalcod in tire diffsrecs col viral inhencvty betwsecn HIV-I rod HIV--2
Methods: Fifrees bloadasaples hive beenra ticed from HIV-2 infected patiecnts who
itltndodIthr e i frest Porguaese Hospital in Lisboan. In some 5cvesviiruses were isolated
iper yph or 1looad msnosslsarells (PBMf 3 Vf genes were camplifed by single sound
aolyoserire cha inraeaction (PCd, irid the inmplified productIs were subsloned is p1 steid
p5Kv (Sir it igorrefSevesrl clocsofaf ch strair werc sequenced by the dideoxy method
of Sang es.:basecse osher jgense'were closed ic plrasid ph P3 (invitrcogen), aides nested
PgR Pfronmf5BMC. isnd sequaensccldlivshe dieaav rsechord.
Results: Aas i fa i r aci sequences ofV faproteinas item HIV-2 confirmn the existesce
of putaticc fanctiolr i solive, csira cterisorcsof the Iraticirusvsuvbraily oh the setsravisrusW
asa abservcod is preserce of twa very cconservcoilregocscase with 18 a..sis the astisro
trisus ofrvsc this predictoil protess asrd thre othser with 9 ia. in rhe caisboxyl half oh the Vif
prarsin Funactiosnal srudes itsbeing ma de re order rc assess the significance of these cegions.
Conclusions: In Vif aroteins we sacs favnd cosar vedcciotifs, specifhc of HIV-2, which Wsay
e'xplair rho'dits'snces bertsesn HIV 2 nilHI-iIinalectio055
IsraelBarrhona, aculty ifPh' rm5'iry v. oxfhLrbun, Av. furivAsradas,1cOO fIs boa,
Porstuigrl Telepihste 35179f 33064 Fax is I 79312 12
Mo.A. 1037
EFFECT OF TAT-PROTEIN ON T-CELL EXPRESSION OF ADHESION MOLECULES
0. rtairo-Velez PfL Vilis cask ite V' P tar r y M"', Fesyo EM-*'. B berfeld Pi
'Ittrs acop rho s'y acbrcstoiyKarlirs ka Insatartes'apital Microbiology and-tumor
BoogyfCecier, K,rrc ins 'hrIrst'its'e
Objective: Toiran.iyze tie efeiof Sco'i5t.5 I, 0-y prss"d ratproteon ot hc' d'splayof celI
idhesion tolicclea CvfAM) 111 cells.
Methods: Tfe profcilesepreass h f 10differesnt AM wry deterosrned on juakat rnd 055
titnsiaicftd Jrat itcells (J-tsr)by IAGS ana lysisuing ir indirect rosmusofluosescerace
methrod oncecllorspersiors acd bf r. ssc5yaoc hens ary vsicg so ABC-aer sx dave techniqsie an cs's i 5l preprts
Results: C'lisrI ies errs c cinl isexpi c 'cicn oflsvesril CAM wcr e obsesved betweenaurkat
arid Jutatcells. Iris ored I sion o f (D) I,,3Ii, 28. 2)9,.9a, )b, 49c, I4e,'t9f, 50. 54,
58, 8 1,102. rs we~ll5 as Ir [A- I wi.evidosni an the cillvurfice of J-tat comprased to
Juarkat. A csrevsacs1ng nsracellvlsr inrers' Ni nlyrab sevedor C[[IIlassied CD31, but
amaredod Is' ssc' in cytoplass's starcicg iafo[3D8, 0.138, CD'39crod lHLA- Iwas cvidccitflrawseersfcxrsessin5tof 13, [[3, C[3'9d.rsd 620 in J-tat cells mrs
dcrease iso''Isrslairs' to Iraikit,
Conclusions: TheIrser sveil ldieer esn tire expr essircoh [fAM in J-tat celcl op arsed icr
usr[iicells csad rsflect sodulascary efiects of extec'rve gene ictic rireslit scirvted cells
my sythis acquire ahig'her alhsivens vtoothes'scells ianI exti scellulis mittrix tihercly omodulatinagthe'tissce 1omn 5g isn2disscnatn if sI I IV infccted cll
E yr icr sVelez. lraasocia this Igy Ibra ryar inskaI nhiIs tiute/ fHospital S57a 76
Stock holIn, Swedencr Telpopnesas6If8-/29'4689 1F.rc46 8-34582/f
emi.0C Velezao rat ikso

W"estern blot sir
Conclusions: Eharsc,
sohsbition isot I
tis isnhibitionsby n
aD (d thati rieeeffects were nor iLce to cianriges ontie evcccfe't.
oIi 'Is levela of wild typ e sec can rafhibit kiIV- I eplcsties.)ibis
t csa egolstior of tar expressinby rev The misanism0.55oe._rtheycstody.

Vern Natar sir N 1 5 35 D(. AICPO. Box B, Buisling iS do 12Isrderick, MD 2/ /0/
Mo.A. 1039
TRANSACTIVATING EFFECT OF VPR ON VIRAL LTR AND C-FOS PROMOTER
Valerie Philipper. Z" Mar sdrTi.L ieM. Es s'x. tDepartmrectrosiCacor Bielegy. Hvivard
School of Public Health b65a lootisageoiAresye. Boart MA 02/ I5
Objective: To determsnoe the ailtyof Vpr proteics'ofs'vesr as pimts s ct av rsto c
vate tire expiression o1 thvirass LTR Pndicil'lularisactis te nvccvedl ir celcl5ysc' proggesion
such as c-hos and (jn
Methods: Fibroblairi,. 's c gi srd V el 5lioeaver e tias r6'ter vsifh Vprexpressioncvyes
tossand HIV LTd. Ia'os or npirometes- insked re thre CAV (chl r cphe ciio 5aetcl
trashers ae) gene The attrartiosof promte rsexpr'ssonins. detronesoby CAT fI ISA.
Results: Vpr proteinsversa SIV/H-IV2ario ible to actvarte the expre sio of is HlIV proir or
er.Vpr saf HIV-1, HIV-2 isnilSIV is so.rtva ted risc'expres sos of humns 0c 1ev prootr:isar
revolt mrs observed is heriseei otypesh oi els. Inc cnirs 5 Vax oI SIVo 5 rod HIV-2 idi
not trasisastiate neithier the HIlV I promo'er norastire s-fey pranotrs No tr isictr art or of
the s-lot preootermrs obyesrveil with asyofrits' proteis vwr'testoeI.
Conclusions: V/e demonsrated thre abicyahVpr pirateinsa hromfHV /2isni SIV to activaste tIhe
expression of thc viass5Li P.11he expressior af tirece lviaisenec'fs es5alsite Iby the Vpr
protein. No triovsctiatrcg effect mas alercv'd los the Vpx proteics I HIV2 rod SIV
Vale're Ph llippi siHdirarid Schoolof PubliHieath 665 [Hvuis recorAvernreBestoo. MA
021 151e1617-413032 fix' 612-/3)-8318
Mo.A. 1040
IDENTIFICATION OF CELLULAR RECEPTORS FOR HIVI VIRAL PROTEIN R (VPR)
AgyavooVelpands. [Miifssi r gram.S.. Piruog. M.T. W I arcs.W.V.. Worimer, D.B hivsiscsty oI
Pennsylvania. Departmtostcf Patihology Ph iadelpias.PAhiSAs
Objective: Identificties ofrIhe host cellIlar protoinsneractong NwihHIV- a pr using Yeas
Two-Hybrid system.
Methods: Vpsr-orneracting piotesns were ilentifbed using thetheist T./Hybrid sysrenarod
smosuneafforrty celusaTwo hybrid proteins Noeewide: one 'slbetwen tie brildrag domain
rod the known pmotsitsn Vpr rod thae other msa 5hIybasid ofhehoctiscion domansod ifhc
unknown protein from thre chiNA libsriry/of booas PBL~s. Intesrittron Ibetween the but prtests and the unhnownms aisvsryci her thriogh detectiasnfof btaart sse lr p duo in
additior to the brirdisre aelectison. Positive cones were screened mnotiplctros tot sctivaty
ard the binding protoins more sequenced rind re lonod iore expresseta vectors and inslazed her biologiclact iratsr.
Results: Following repatedascrigs, me milentified five d2fferent seucesfroststhe
cDNA hibary rod the sizes rnged irons 0.2 to 2.1 hB 1 hier sequencvs were sirslyzed
against the Genelsirk amrteicv. The binslogical cacy of these preteis were assaysdilor asnd
the results mil be dirsmrssl.
Conclusions: Vprshhr been Iown to tirirs e te hroas the rcytoasm 5toiuvle05, i0c0eisinag
the aisal r'eplicitiesnis sondiaiding sells especiallyrinmacr op1higes soonoc~ssacIt basbees
described that apr iniluces cli differentiti~ron asnd inhibits celIpasliteraisn These ranctions
suggest that apr irts ifsough ocellulis evesnts rstirsthvi s5ravets.Idlesntimitson ofth e
celluiar arotein siiciledte the' apr fornttera coold re a postentialrtaigerforat air al
developepnet.
V.Ayyrco.142/fCmmis' Bavd siam501 oh Stellar-Cfhi'ce Liarar it '-y Phone[phone]'5/
Fix: 215-523-9136 Vnmal: las' idrso ilo ccl opens cdv
Mo.A.1041
SIV/MNE NEF INTERACTS WITH RAF- I KINASE AND INTERFERES WITH ITS
SIGNAL TRANSDUCTION.
Herdecher Gisela'. MoodI. K' s sot.,f.W5' BOO3P/SALC NhCI fL PHOanis aOb.
Leukocyte Biology NO. fCdtDC. Freodesrich, U.S.A.
The tf gene of HIV and SIVf 5 cme into 1crs a a r contributor- to thepthaoges'e
vis of prioratestmnrific iesci es.Sevesralies ml evidencsagc't tbhat Ne titer/crcs with
signal rady tsr o ifctrod anlpovsilay ever rn bystandrils. We haceaayz'd tire
motrsaction of SIV/Moo Ndf artern wi'bh seer a rt atsns ir she sgal yr r t 50c 5 rnetwork
usig so-precipiratios If proteino expressedinthre 519 b 5 us a 5 0 ystem ss wmc asi
manmmalian celiv.We Ihace iseorveilif thataNef ais IaPsI ntrtc5t. Ihe rateraciotrssbetw'een
Nef and Paf- I ma depe'sdeet or Nef myr s roylior rod irorvan the hinssre donsaso of the
Pal- I pro'eiRat- p if r t yt5a"rsminscosei tr sisse scinae wmlch isaiated inrason e
to Pay stimulatiron inddistirnmaphosphiory ates MAP honr e kir 5 0 (MAPKK - Mek) As a
consequence ofdRatIsctivatorrhe imme'diste s'.rly r'esponse ofhAP-aIdependentttinsrptionia tuirnedos. PRif- I ima nr 0esldrot poirait rho sgsalr tsrndo tirsneorwork a
knocsk eut of Paf-aI anviy by sotnaese el gos or by dr ia S snegia' notutattsaiorbethilto
cells of all lineges tested. Eapsression of Nef so mmmaiman cells rlso iown-mraSegulatio
oh signalinmg somponenscownilmsatream of Pal- uch as MAP fkrit 5e nilAd- I isd NfkB
dependent trascsrimin. Is aontrs at, SIV/'irnNef with inrF.P18Y m15 it i 05aticat itogoali
cmpornnts dowroire si--rfIQdif I TkS.-.stirrer-L_,.. _-_ee..e.._S.-.sL. p, bnDa t_.

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