Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Tu.B.2164 - Tu.B.2168

Tuesday July 9, 1996

Results:
Bio-Rad Cambridge Innogenetics Ortho Syva
iit~ Ny.N >t 4 1;I11 4458%446(5 0524"t45 0?9 41,
Conclusions: Assays performed within similar7 parameters to those already on the
AustrarianrisMarket.
Ms. Susari J. Best, National HIV Refer ence L ibOr 777 7 Fairfeid Hospit7),Yir7':r Bend Reid,
Faiirfild,3078 Australia
liephone 61-3 924)52450 Prx:S61 3919241452 eal 5 anr~e~sus,OZ,10
Tu.B.2 164
COMPARISON OF TWO COMMERCIAL KITS FOR MEASURING HIV-I P24 ANTIGEN
LEVELS
Sheriock: CHf rtasL r KJP BC (Centre tor [xcweece irs HIVAIDS ard tUrisersity of
BrtitobColumbi 7 V 777(river BfCirsnsda
Objective: To compare 7,tie peidforia 705 of two cenmrnerrci ELiSA kits for' reasur'ing tire
concerrtrartion of DIV i p241 intigen fp24) ir se~rum before rrsdarher irtrsursercnspiex dinMethods: Previoirniy frozerrnrrat frons HIV infieted nd low-risk HiV-negartive patients were
seiected frosm7inia r777oe eciverd 70the daorstic viroiogy iab.These were tested with
Couiter's HIV I p24 rtgen kit, both befoinesrsd after osmurne rompiex dissociation (ItI-D)
wit), CoultrrICD Prep kit, rs we)l rs witfi Org 7000 Tekniko's fOTf Vironostikr HIV I p24
antigen kit, rseobsefore rid ifter- ICD withr OT'n hase dissociation kit. Couiter',, lCD procesdare h~rris 707070 00epti rtafisheOT kit.Vsfrerrsufficierst serum winstavrirhie, dies7repanrt
r'esrritn werserronirmrrrdr with the rtranrrfrtcrer s neutrarization (treat) ossayr 1 he kit insert
instrurtiorn were foiirowed for ali testirr.
Results: Of 2h3 DiIV antibody- positrve se r toted before lCD, 152 (5B8%) wers positron for
p24 by both kits,43 (24%) were negative by both kts, 4 were Coulter pos/OT neg (all sorsfrirme by neut)5,iB wse70 Coiriter neg/OT kposorrts not drine; al) pens y botfs kits after
CL)). Whentssted rfter ICD, 21i7/worn positer by both kits, 2k wore negrtive by bot kits,
2. were Couiter pos/r)fl44so (both ronfi rood), I was Coulter neg/OT pen (corrfrmrod). Of
50 fHIV antibody-negative sera tssted before lCD, 49 worenerrgative by froth kits rod i was
Couiter rseg/fCT pm (nrrt renfir ored) Abter lCD rii sera were negartive by both kits.
Spearmari',, rho (SR) for p2l ronc entrations~ between the 2 kits was 0.9 i15 (p<.0001i)
before ICD ansf0.91i 7 3p<.0001i) aher ICD
Discussion and Conclusions: Nerther kt has good sensitivity wthout ICD otftoagh thoeOT
kit arpperrs to be mere snrsitive tihan Couiter. With ICD, bosh kits ario squariiy sensirtive, arrd
very firghiy correiated for antige ors erntr itrerss,rnd specific, rthough tise number ot negatives mrs umali fora sperifrcity arrls in. Tfin OT kit is mote 'user friendiy'' with 7000 ios lCD
step oar) more premixed reagentn.
C.H. SheriorkVroiogy-i1081i Burtrrd StreetVoncouver, BC,V6Z I Yb, Crnada
Tei. (h04i 63 i -54264Far: (504) h31i-5421 e-nmaii: rsnoriorkigstpoiulshosp fo.ca
Tu.B.2 165
PRACTICE MONITORING, GUIDELINES DEVELOPMENT AND PERFORMANCE
IMPROVEMENT THROUGH A CANADIAN QA PROGRAM FOR HIV ISOLATION
FROM PERIPHERAL BLOOD MONONUCLEAR CELLS.
Cinensens, Chr'rtsrre, Fauve), MH krborrtoirre do sante publrque do Quebes, Ste-Anne-doBoirevun, Quebeco, Croada.
Objectives: I)( To moriter rod improve the performanre of Canrdirn diagnostir and
r'essrr'h aforatorien ssirating RIfe from peripfreril biood msononurcear reiis through r voiurrtar'y QA proegrrns. 2) 'to dseter'osne optinsa) conditions for DIV isoirtion.
Methods: Prrfiiry parroi 35) ronsinttig of biood saropien were shippecf to if) ilrfor'.to
ries.. Participat er se to et thesanropirs rod to provide detrais of their protorel.
Ev~irtrein of performnrce wars aseno n tfre ropacrty no distionrrish HIV positive fronsRIfe
royrative srerpie. Pr otorroinwere7 reviewed to investigate factors ieardirsg to iradequate p707 -formanrce. In rdditiorr remparative exper ins were rorsdrrted70 order to develop guideines for HlIV iso)ation.
Results: Tbeoerl oa ionrto of participansn creased steadily since thn eimpimerstrtiers of thse 577 oyr 707 fi ri 56.6 %,roin99 3 to 88.8%. it 13995 Peer performance(0in nomeelrb-.-Or.7t _,, +,,..., 75recicdf 77 rbr +e f70000rinr oir.., n mdirrrdelcrt,, ikrbor,r
Tu.B.2 166
A ONE-STEP SOLID PHASE IMMUNOASSAY FOR RAPID DETECTION OF ANTIBODIES TO HIV-I AND HIV2
Hiler C., Cambkne, Brgte. Boehr inger RIanrsheirssGebD Mainnhsi07 Ocrmanrfl
Objective: lb provide a rarpic) rod relirble solid rhs nuo~y for- DIV rrntbodeos 15a1
reqcrirosesiy mirimal labeor oriiripmer 0
Methods: A (0000777 carrier 7 075 ()caltaiveI7 V1ro7n 77 ~sof rsenc0elf rntbodi05 10
HIV hs rsboon developed arid e710u7100.Sse of eu~ie n olidari '07ld77snell asnorizontal chr'orsatograpfry uporn s777790appliratioerr he othorsedryv rcrr'ont car r icr
allows a orse-step preredure.To roolyze s0er70 or7pli 777777hcc u0erpplies oppro00im7aels60
p1 strmple vuensand rHer 15 70min7readsthe 70' ult A fruilt-70 procredurr-l'costrol c0ads 10 7
pink linc when ru's correctly, rppear rc of7asecorsd pink lincndicates 0r5(ec ofiretivean rtibodies. The reaclion prinieis r doubltieg1700sarssd'n-icr, e07 7711 o'detection of
all typos of DIV specific mmunor'iobuirns
Results: Dporn rnititi rnalysis of performatnrcs of 1)he novelinnrnoasa000chwa 70e
comsparisono to tfic Erzyesrri-To-I9 Aroti IHIV I +2+7 scrbtype 0 (a 3rd genrartiers FA of
Boehsrirgcr MaIrnnhim) Ifis foloin7g 7creuts rue been obtarrsod:On 6ri4Inet utcrr10r proe-e
lected positive srrsrp)0- Nf w ichicludsed SIV- mjrpr) I V" rs weillis few DIV sub15350 0 infoclions rrnmplte 707reerreritfretweero the twco tests was obtaiind Specificity 0on
rrrseiOcOtri blood donrs (totaI16170) fr ormrEur ope 77n1Africa as99 077 efrrdto
Oimcrnblot Comforrdge Botech DIV- I Western Blot). Sampsr froms hospitalized persons did
not shrow signifcant differeonce an comspared to blo donors.
Furtfhermsore0,077 I89convs-o n paner ods m0e0r ciallyiviru til the0ra7pi7075770n5,t7
eovenred a simir sensitivity asth~e Enzynsun-TostH 7 ~ o-1 1+2subtype 0. Model oxperiment7s o eriorcr1dilutos of p0siiacenorri 7s wellat nr-ti-HS-IV smonoclnaI li ~odien of 0e137cr
IgC crc gM 0nat77unreshwed (0777)araeres suslts fc'r tferapid77 Imm77uns0010-th pot
rsnetric ElA
Conclusion: Poe newly developed visiayre17 a7,d 7rnp,0d070ir770 ist ses t,hghlyreleanfle orsd (177be performred wth107 l boaoyeqciprrict771Resuli reon 70avaiablre -within
15 0770 after samt07 p lcatP ion.07
Dr BrigitteC or7bke, Boeoringer Mannheim e Hrrf, SDept. AE-S S I So.are!16
68298 Mannheim. Gecrrrroay Tel:+-(0)6li2 7c-25962,r': +~ i0)1r2 -759259
Tu.B.2 167
HIV-lI SEROCONVERSION AND UROCONVERSION IN SUBJECTS AT HIGH-RISK
FOR HIV INFECTION
N.Vardinon,.Ycst,Y Sein, I. Zeidin 9 LDickstci, M. Burcke. Ie Avia Medical Center rod
Travenol Laboratorien, ISRAEL
Objective: (I)( To exarsine the urine of krnwnFHIV- I snopositivOeniubjscts for the presnce
of antibody to DIV I. (23 15 study DIV- I eroonvernion rsd ureconvor ln 7n scubjects 71
high-rink for DIV infction.
Methods: Blood wsnexaemined by FIA (Abbott '70 generatio07) for DIV 7Irrtbodsy0770by
FIA (Virorsostik ) for- DIV I p24 antige7.Sir incspr'ciesn were tested b0a0 70-hrouse FIA
(Med PIA), whore reaeuno antihmnIG(im)w usiuted 7r 35as the conjuate. SODIV
soronegatrve subjects aod 50 seropoositiveoindividuatin norv000sonregtive rod pen-live controls resnpectively The test group coespisem3cdividuans aehgrisk for HIin777t(170. A)l
positive reoncrts (blood/urnen) were cenfirnec) by Western flot WB3.
Results: Drino specimoens of 50)513 enor 777 ive 70eaclod noe'arIveny to Mod-EIA The crrine
of SO known nor opositmoon nilrendted positively to Mod-IAT)-us she nernlsitioo d specificity rr subjects of Proown serologic stars re70700. xhree of 1170 high r in irvidcutinsn'so
were seropositive arrd p24 antigen positivelo rieacte00positively 777 Mod-EIATmoetfiern
wore penitiveoino Mod-EIA rod one of these gp I160 ur oponitive fry WB. burt 'remaned
seroroegative at 3 070nths.
Conclusion: DIV-i1crocover'nion sony pity a role 70 diagosinscr3 recent infectior. Subjects
with recent serocoinvernion olno exhibrt rroconoen sieri Those casos whore 0771y ur'0(07007'
ion is founrd should be closely followed for ponsiblersno conversi077.
Cons Ncrith VardinonTel Aviv Sorrsky McdicaiCCncr71776oWoizrsar 7 Street Tel Aviv, Israel.
Fax [phone]
Tu.B.2 168
POLYMERASE CHAIN REACTION (DNA-HIV-PCR) IS NOT USEFUL TO DETECT
LATENT HIV INFECTIONS IN SERONEGATIVE AT RISK INDIVIDUALS
CriteliiFP7ancesco,Cnssr 7 5 Ba77 07 C2, Res I, an M Prait4P Ascain P 0700777
F6, Cirnquograin, 7, Crnell PE, 7 liMS P FrrasnP Putzeic V Ci nic of Irnfect &Trep
Dis, UioBresciain(Itly):7101stv.Ifcct1i,,3o 33 r;fe lr muo15n a
Spedoli C ii fBresci70678,9, 151 gAdc o evcs LclHat Uisn 76,19.
38 &5 i4,Regionr Lom~bardy
Objective: To ssviuate the assfrrlnesof tsc h frcntgrte 4HV-DNA insPBMC to
detect latent infection in nor 07707 live sucrtatlnk
Methods: 007ortof 71,ne rvesirj(sit0(5 fo r IIi 077ton)dn ryg (ldicnDA, no:b es ei I O p I 53 e ntts, I -..,.Icr ly ci soic -1 \/.-,t,,,70 ot c -

293