Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Tu.A.2018 - Tu.A.2026

Tuesday July 9, 1996

pheo eois reatedltoprogeionrftHIV ifectio tsell r t irtnluerncd by (oubcinical cror' ) pporturit c nfecns.
Chhi I r N9" lP Bx5000 )0KiEnrctete Nother rod Te 3153872443 Fax 31 3
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Tu.A.20 18
MAPPING OF DISTRIBUTION OF HIV DNA LOAD IN BRAINS OF AIDS PATIENTS
WITH HIV ASSOCIATED DEMENTIA
Fun iii aRotbcit K [pler MH Cu dkirK. Menz S, GonzrlzJ Sripsh ik P Department o
Pyhiatry rnverity of MiamiSchooofMetrcireMinmiFLU.SA.
Objectives: T entstblish sinatiorofelevated HIV DNA load w ifhDIV issocited demenr
ir i rsof the hrair rvolvedinroyritve notor forction
Methods: I)A retrospectinepost n' ten psychiaOr ionutpsy specific forcogtritive-motor
disordOer isrradrrdizodayr t wai 0sed to diarooe HIV aisnccated dorrertr. 2) The raio
of IfyFNA copiesper celilular n r wisdeternend inispecirrersfromirseveralni co
orirt rri oca rtion y i inearresonre 0uatitive PCR technique.
Results: Or results ipyr tt hit tie tttV DNA cpy i high rn penmens from hrppooimpus the dititironrrirges fromnIcopyper 100ccllulaii B0me ir cerodellrcm tomore
thanv200 cpies per 100per oro ' rin ipp cmpu t on asswithr HIV asoociated dementia
The r n ber of inmpres irniyzed is ti t ito l t ubect to stt isticaltarrls
Conclusions: The risits arer ni et i t h or typotfesisthinHIV DNA toaid in etevated
ir theiarersinoved c gntve'iotirfuit
A ke vvedgrrert Thisreeacissuppootd yNtH nrart DAD'787.
RK Funmrvirlliot Butrlding 22h, Ui versiy f Miami Sfhoo fMedicno, 8003N.W
tf Averu, iramiFt 331 n6 t,:302133i917; BAX.3052435550;enil
rhoii va wsn rd i ce t rii i 0 i
Tu.A.20 19
CONCEPTUAL AND MATHEMATICAL MODELS OF AIDS PATHOGENESIS: TOWARD
A UNIFIED THEORY
Roper' Whitiker. Ph)D Prime Dirc ve onsurtingB PB 0951, uerrevttle CA 95346, USA.
707-525--476/
Issues: The preyailring itaraimo IDSlpa thioBen illed the viioitgBcai hypothesis,
argu~es thait v irlittitestich Isi yrto titt r.iepti aity ynyt iity nettltiopisin, emerBert viircirnd viiaitoId ire ii ttcit to plaiiithe paithogeeis f AIDS. However,
other thypoitheses hive tbiei pripIsed, such aisThI34h 2 shifts, ipoptos o, rotigenic dominanre or copetitionanindtue r riid3omviiaescrpenoutrrntholes onthrenmunoogiarepertoire foloing cel l0s, ait iecive cell ft DBor CD4 phenotypesard inhbitio ofthe icr itirrert aor otir itor of precurno ell.D esehypothesesvaryroore
anrole-,inthecwayttoy proIoe that HV retes to D4 Tcell death,0iminoRogialdi
ropt or mlanAIDS pith orees
Project and results: C o~i onpua indmthti i oiiltoaiiOpir0pOsed, developed Cr om
previouiy/tblihi' wormcihat sowinirvirolgiclrypoti tobe iocdquate toexpiain
the ltercy, mnlo g ialiiamayr. ro ciniadyrario f AIDS.tc itlterna tive hypothesse comrot nd oi'd i.0 aie'atdressed, ruhoastheripeuticvvccioatorthe
potentileffecsoftiecoi onWithHIlV thiere5aothptetweecinioalsod Liboritory
drugyresistroce, adter ecco diseaseprogvi n Airve torda50i00lusve and
imorrr clrtheory otpithoeesos s c.ad riondthecodits orhiseasrogrpessoo
thtornytheorynmust rdres ri outied.Tthisoricaltrend i ADSreserh issessed
in viewofthe ibovyr
Lessons learned: Itrs apar~re ini 0a mc teiing your prtog'yesi orrtehsbeen
simplistic fnt o prad thatmrr ro hiticaited anyes rnd reserch0arereqoired,independert otpolticarad ridvatetofiniaicniderioo.Further.5 in sthat CD3TcelI
loon ~yostilb telfbeiadryrite toexpinthe clinia yodromeof AIDS rod there isa
need forainmri crr~pSsiv ondrdstondtry f the dsease.
Roper t WhIta7cr, Ph.D Pr role Firectivi Consuling, PB 095 1,oci r cvi le CA 354356, USA.
702-525-47/
Tu.A.2020
THE PROGRAMME FOR STUDY OF HIV PECULIARITIES IN INDIVIDUALS
BELONGING TO THE GROUPS WITH DIFFERENT PRESSURE ON THE IMMUNE
SYSTEM IN UKRAINE.
KordiorniVtly A.',B Oseoko Yu. I -o v D.'.A 5 r cr7 5:.T hoots vi N.****
'ontit ne of Moleculr- iolo y andGineticsKicv, DOkre,''Arti-PlaqoeStbion, COdessa,
Ukrin; 'onttute f [o err 71,Kev kriore; ''"NaioralReferecehiaboritory
Kicv. h Sr it e
Issue: As es. o uiue 1, " nc Urine h0. e areconditons w irder the
stin ryeyrtivoressu're nheimnre systei o ft tooio.Tteref red bth iofectier
wth DIV rod ADS mourfho'ti on iinlrerdy in irodehorienorgayims.It is neon
sary t krow thetftV o u io r 1 crairyinthey r pSwith dhferent pressore othe

primory sequence of provirit DNA. Scci0inand ito ryestgtyron or I tV ro00 lri t e
waO foresceonto cairy out in the regon with purt i ~ry ur u it e ecoloynclrstuto 0
wel as among ewe Broups of druy 00dcrs.'omitaireousvlyi sfreseon the tudyoftre
p0orbilities of circulation offHtV undeor HIV rsimirpathogyin i I riio" y hcayii v
the dietogicat materalfrom She pitsotwith ryp r it irtte tatr r iAIDS ob.t ordir tIhe
negativercoolto of immune ting.o
Lessons Learned: The retwork his hoen crerted for pirtimay oleio ofr h i 't ioroen
the biologycamateriatas wellasfirthoe nriotrmaion S bologiSormteril
envestigatoscairy out persistently rod the ii'uitsr incumuit ingtotiee reoapering
wilt do presented io the paper.

VAKor d om ISO Zdotnogo Sireoct. Kicv, 25243,r Ukrinei.0 30'eptc
Faux:+ 380-44-2660759 coo i:oodovageo ct erk cv i

3.4-2665596

Tu.A.2023
HIV INFECTION IN VITRO ENHANCES THE ACTIVITY OF SRC-FAMILY PROTEIN
TYROSINE KINASES
Phipps. David 1.1, Read SF2. MIs GB3. Branch D[.R3 Plryfaii NeuouieoroUritThe
Torent Hiospital; 2 DysionoftIntectious Doserses,.Hopitalor S hildre'.T i nto
3Motecutor Bology MD Ardersor Crncer Centre, v t oTexa USA. 3Cr od irn Red
Cross SocietyTor onto. Canadu.
Objective: We examined the cfect of DIV iofectiono oeincrtyro 00e or P-fK) ctivty to deter mine if PTK activity cold cntibuIeito t ho U Vie ted Shroicitmmroe systen
activator observed in ptirents rhoeted wih HIV
Methods: Jurkat, a CD4postiveTlyrmphocyeielie 00sWinolectoo wih DV IB Kr r e
actvitymiasdeterieddby in viorune opec krie aismys"mmopc i'r ir
antibodies specific for the orc-faily PTKs toSS. c 03p59froad ph.scPF-K piote oinian
tialphie Protyros 00 (ptyr) were oetected 07 we sesnibeotoryg"'rhe r oeioy p 0 D'S
noteton io DIV-related PTK amtiviti on widetermoired usig771 20-treated (JurSit n0
DIV-inhection of (CaM I1.6. o CD4 neg9atiye (or kit-deiedyellne.
Results: Cells nfected wth DIV for 23 hors echiised inrnoede es ftsi netpo
phoryation and erhanced PTKacivirty wihuviltrerd lovens tfexpresso ffa iy
knises.The activty of Ink and (70 wOO enhirced wihin 30 ciuto f ioeonO. DIV-relted
PTK actvation was not aifurctiorofthe gp 2 /CF 3nterci oe ndoccurred io the piesence of 10 oH zdovudne inditirg that revorse ror nc-pir c iin3itit Sirfte DIV
gnmeis not required000r"enhair"ed PTK activity
Conclusions: DIV-relited PTK activiro is aroponsrof the clo teOiiy3stiges of toe viru
life cyclepossibly either merbre fusion om vi r umoaiii Fhesei"sit inditttsSorrily PTKs play i rote in DIV-relted immurne artviti onrod prein'ct ifit cothinced PTK
acnivity coay be measur able io patients hortly after iofectio o.
David J(Phipps, Playfair Nouroscne Uin, Rooit c H 3 Tt e T i ni
Doopial (Western Divison)3)9 Bathur st Strent. for ocioC.Oniar io.Cnli. phre 36
603-5736. FAX [phone]
Tu.A.2024
EFFECTS OF GAMMA RADIATION AND H2 02 ON HIV REPLICATION IN PURIFIED
CD4 LYMPHOCYTES
Xo Yrndong, Conway B., M otaner J(S.GC ' S gore sM.V British clonb te o r
Excellonce mm DIV/AIDS, Vanoven, Britis o o oni i, inaid
ObjectiveTonconinue onr tiesfithe effects frcimri dit or o0 DVr"'plitio i
vitroe
Materials and Methods: 0CD4 and CD8 cll' fomn 007 gi 00'0onowere0 003arateciby
cell s00rtBno nin ollordait super para irn oet ic mcied 0conjuatcd with c lonai ant
hocoar CD4 asnd oct homain CUB ontibodies MACS. H tory B oec Inc.. Sum r yO o'USA.
Isoted and renombiced CD4 ond CUB cellsaswellrasiinoparited PBM.. were exosed
to gimma cadiation at doses oftI to 200 c~y or iroted wif 35mnM H2(2' provding
oxidaive stress equivalentto 50 cyoCo intolcls wore ptocessedinopamailelccxclp o
exposure toraodiatiroroor D2D2. Atl noliswore orentinmilated with PDA-P Iv(I i )fo-23
hoursihen infected with D'FFVIIIB(M..10.0 l) rod mitie'3 culturi ohr '5 iays
DIV reelintion wao 00000sed by o24 intigen lcuels iin utr enta n ts5wIhweroe harvested tioenoweekly
Results: tnouor first experiment, we wore ucible5oe stbIish0impodictive'cunomre in onrim
CD4 nell. In cocirast, high p24 intigen lovelswere ineorured ortry 13 0 ellontxo sdt
25 cry (17.8 cgml) or35 cM D2D2 94.3 nrigm n r mmecord exporm ientp24 arigor
levels inCD43cnols rod urseparaotei PBMC wore ircremsei to512 13ffolowng0piecopeoure So 200ncry Iterestingly, n sticorlit Sorr I asobr vnd irrecorbined CD4
ord CD8 cnos.The eofe cof D2D2 was anly toicsont iPBM sareromisd 0CD- Inmm
CD8Bcnosnot in isolated CD4 cclls.
Conclusions: We have confi rood or preyios bsemviti onIftoe dfe nCto cgatoaradia0
tier DIV i-opication. Different effects mc CD43cellmreobicd CD43anrSCDBcels ii
unsepaaed PBMCo ore cotnwellexplaired arddo io 00ctlyparalemte n00 cidmo yrn
krowr oxidative stirro ms D2 02 Thus the mchaim inoderlyy iouboornit 1ion a
only par tiaty relate to oxidative siressrod r errains ndr study in uroratiiiry

Xu.Yrogd og. Beitsh Ceoumbir Contsic for EcSc e oinIV A1535 6/7
Vancouver, BCV6Z Y6TeI 1604 682-2333 Ixt. f 7):fnc. 6 3 63 -52/
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Amy op bitstem. driieid Iics
Ther' reitwo giops fdrg d erttyeofrupdsrongrid
ene cot ill those faitos, thi'parlori
odefcirny ciiarly expreso sed.Iti
mory or'quencee Ifthe irorio' wil
ofdifferert fictoms mnthe in oe
vems using the stand ird imcornorn
of t ido,-i isabe usnd doper/dneo
pl nfo _ted tby DIV. It needoco taSie
of the icmuresystir 030 ti m
nfect rion gensrisr mo m v05
the coiplcx thrimctom Meticaily

l 'cy3ditibunignterristaly parim arlstatndso uS.
(S iO cerily disigihirg;sepraing they ose iho difieoirvaisinwith orionsirce.An the euSt of tih' ro
p ppultimn0in theconry displsothe sorgsof immoc
lil~e tSwont Shtot iSderoticilDIV with ohicged pmiS irclte if te vi op of popultio with the toessuie
stern. Ioweri0g She effecivnssonf mcneaing HItV carvymse diags otic lo osidle Listlydifferent schemes
)nhltV pecuiaiisaditheimouno syioem stiiiinpesno conicerion that missodovrousosippresson
oUkmainecammcreateotho'centers offormion of now
ni rc thot r droso.Th tit why She pmrgiic hos
the' mmtcuaatioi is 0 paid So detormcinaion0of the

Tu.A.2026
SPECIFIC BINDING OF ADENOSINE DEAMINASE BUT NOT HIVI
TRANSACTIVATOR PROTEIN TAT TO HUMAN CD26
o lio Banco, Isabolle Mai e Chistan Cit edrun, Ft ens Jo (in5 5 Bernard Kr u t nSAir
Cr Dovareosmac consitus Ponteur, Unite VIC, DA CNRS 157, PursFmani
Objective: Aderono deam 0 se (ADA) rod HDl- I trio r tin it orpoen Tin hove beer
reported to bicd no humnan CD26. We ne sinie the specifciy of ZrO is ADA brn
to hocman CD26 expreosed en the srmfaeo f cur ore ls
Methods: In order to demonsiirate the spe Cfctyof1such ben/ny mnder nivelo ro
CD26, i.e., whom expreosed ontcelotturface, we etnisfed so r me elliesmexnopes
iron footed Chuman CD26.Th o experimenialystomiroomsanousine n i so ADA
does not dond humor CD26, whereas humanndroevne10AD3Abidd

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