Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Tu.A.2007 - Tu.A.201 I

Tuesday July 9, 1996

oad. Similar pheonomenon was observed with bitherapy (AZT+3TC/D4T) and may reflect
of the viral load decrease. No modifC ition of the Th0/Th2 cell subset (CD4+7-) was
observed even when viral load signfcantly decreased
Conclusions:7 hse first results suggest that significant decrease of virus load induced by
ART s associated with a signdiant mobiizatiorn of CD4+ activated cells of complex kinetics. ombinations f var ous arlyctraton markers will be useful for further investigations
of more potent assocations of antiretroviral drugs.
P DEBRE, Immuno logie C elulaire, CERVI, CNRSURA 625 CH Piti-Salpetrire, 83, bid de
I'h6pital 75013 PARISTEL. (3 I) 42 17 74 82 FAX:[phone]
Tu.A.2007
ENHANCEMENT/NEUTRALIZATION MEASURED IN THE PRESENCE OF COMPLEMENT PREDICTS CLINICAL PROGRESSION IN ADVANCED STAGE HIV-I DISEASE
D.Tot F.," Banhey, D., 'frer\,, Ujhelyi,fE.,+* Prohaszka,Z.,*'"F' stG*'S*. *Inst.
M icrob JolUniv. Me d.Sch, Debrecenr, *Dept. Irnmunol., St.La szI Hosp., Budapest, *Natl.
Inst. Haematol. Blood ransf Iemunol., Budapest, Hungary, "* * #rd Dept. Med., Semmelweis
Med. Univ, Budapest.
Objective Previousl we have ireported (Fast et l.,AIDS 8,603, 1994) on a strong correlation f he titr es of complement dependent enhancing antibodies with immunosuppression
and d sease in early anrid internmedate stage HIV disease In the present work we studied if
the saie assocation can be observed in patents in more advanced stage of the infection.
Methods: J10 serun simples were taken from 30 patients (5 sera from each) on antiviral
tr ent (A I, AZT+ddl, o r AZ + dCI wth a median CD4+ count of 385. For measurin the amounts of erhaninr/neutralizn antibodies in the presence or-absence ofcomplement, heat treated (56"C, 0 mn.) sere iit a dilution of 1:64 were mixed with 100 median
TCIDb0 of HIV!IIIB, and w ith fresh HIV-seronegative human serum pool (NHS) as a complement source (firnal complerment dilution::4) or buffer instead of NHS.The mixtures
were added to MT 4 cells. HIV growth in the supernatants of the cultures was monotored
by the RT assay and expressed in c.p.m. values. An enhancerment/neutralization index (E/NI)
was olculated is follows: c.p.m. va lues nmeasured in cultusres treated with serurm-containing
samples/c.p.m. values nmeasured in cultures infected with virus alone.
Results: In the first two scrum samples we have measured a mean E/NI less than 0.5 (considered as rneutralzation) in presence of complemernt in 17 patients (Group I) whereas in
8 ptirts arn E/NI exceeding 0.50 (no neutralization or enhancement) was found (Group
2). Mar ked differences were observed between the two groups in the course of HIV disease. Clinical progression occurred in 2/ 7 patients of Group I and in 6/8 patients of
Group 2 (p=0.0001). CD4+ cell count dropped from 378 0 163 to 153 ~+ 137 (p=0.002)
in Group I during a 33 month (median) follow up period whereas in Group 2 a much
slower decine (from 358+ 66 to 276 181, p=0.087 in 38 months) was observed. By
con ta strong neutralizationr was found in almost all cultures treated with the same sera
without complement and no correlation betweer the course of HIV disease and the extent
of nertrahization wais observed.
Conclusions: l he present findings confirm our previous results obtained in patients with less
advanced stage disease and indicate that rmeasurement of enhancement'neutralization in
complement containing serum samples may provide data of clinical relevance. (Supported
by the PECO'94 grant to the EJ CA "Developmrent and Evaluation of Immunological and
Virological Marker's")
G FOst, 24 Daroczi str, PO, 4 H r 18 Budapest, HungaryTel/Fax: [phone]
Tu.A.2008
HOST AND ENVIRONMENT DETERMINE COURSE OF HIV INFECTION: LESSONS
FROM AN ETHIOPIAN IMMIGRANTS COHORT IN ISRAEL
Bentwich, Zw, Kalnkowch A, la! N,VWeisman Z. R.Ben-AriInstitute of Clinical
Iranocolooy, KipleirHpit, p He brew University Hadassah Medical School, Rehovot, Israel.
Background: The reasons for the different behavior of the AIDS epidemic in Africa are not
clearl hey have been ascribed to di fferences in HIV structure and virulence or could be the
result of envronment and ost factors. I he recent immigration to israel of Ethiopians
(ET), with a high prevalence of HIV Itype C rnfection and the African pattern of infection,
has created a unique opportunity to address this issue by studying the immigrants in comparison to israel men (IM), irnfected or non infected with HIV of type B.
Hypothesis: We have previously sug,;ested that immune activation of the host in Africa, is
domrinnt r in marrking hi m more susceptible and less able to cope with HIV infection
(Irnmuno.Today 16:18/, 1995). Chang e in the environment and eradication of the infections
should therefore result in decreased immune activation, lesser susceptibility for infection
with HI-V and rmore protracted course of the infection and disease.
Study: Chinrical folow up with sequential T cell determinations, general immune profile, activation marke rs and presence of B and T cell HIV specific immunity were determined in all
ET and IM groups.
Results: I) Broad immune activaton was present in all HIV(-) ET with helminlth infections
and decreased after immigration an rd tradication of the infections. 2) High and persistent
prevalence of specic B idr cell /t V speci Jmmurnty is found amonr, HIV() ETl 3) HIV
infection in both El and IM 'roups i, associated with a siir pattern of immune proile
thou y ET have additional irrrune actvation. 4) Rate of CD4 decline and clinicil course of
HIV in'ection in E after r firat on, resemble those observed in the IM controls.
Conclusions: I) nCan e of enwronrent and eradication of helrinth infections, mry affect
significantly the course of African type of H IV infction. 2) Preexistin immune activation
may have a dual role in either increasing susceptibility ior /I/V infection or enhancement of
specifi imrnity. 3) Natural course o HIV infetio is not determined by HIV-I type. 4)
These obse rvtions should be enlarged by pirallel studies of Ethiopians in Ethopia.
B Bcr w ch, Kaplan osp/ital, 76100, Rehovot, Israel Telephone: [phone], Fax: [phone] I
Tu.A.2009
ULTRASTRUCTURAL AND IMMUNOHISTOCHEMICAL STUDY IN PLACENTAS OF
HIV SEROPOSITIVE WOMEN WITH OR WITHOUT ANTIRETROVIRAL TREATMENT.
Viliega H, CGorbea MC i, Paredes Y*, Flores E i, Karchmer 5'. *National Institute of
Peritorogy INPer). Moxico, D.F + Socia! Security Institute. (IMSS), Mexico, DF

Objective:The purpose of this study was to quantify morphologi illy the benefits of mater
nal zidovudine (AZT) and didanocine (ddl) administration compared w th a group without
treatment. Ultrastructural analysis centered on evaluating the response of H aucr cells
and the presence of viral particles.
Methods: Twenty one placentas from seropositive HIV women were evaiuated. A group of
7 received 500 mg AZT three trimes a week and i00 miy ddl ever'/ 8 h 'ron the second trimester of gestation and in I case the treatment startedin the 3 d tr-irestr and 13
cases were without treatment.The control group was 10 normal placrentas. Convention
ultrastructural methods were used Imrmunocytochemistry with anti bod /y a gp4 dI were performed.
Results: In 3 cases from the 13 placentas winthout treatment vir, pairticles ike HIV were
seen in maternal space, some of which were in contact with trophoblast c ncrovili. Scanty
viral particles were observed in the syncytiotrophoblast. Immunoistoclem cl stainng positive in 4 cases demonstrated a positive reaction ci throphoblast and endothelial capllary
cells. In the 3 cases Hofbauer cels showed hypertrophit and hyp erplas a. In two eropostive children the placentas had numerous wrus n endothelial cells of umbilical vessels.In
these cases hypertrophied HofbLauer cells had numerous lysosones anc some parlicles like
virions Placentas fom patients with anstiretrovira treatment who started in tr 2nd
Trimester were absent of viral particles and Hotbauer cells were consider-ab!y drrimnishedn
number and size, most without lysosomes.The p lacenta with treatment in the 3rd Trimester
had persistant hypertrophia and hyperplas ia of mracrophages.The p p 41 proterr was present
in plasmatic trophoblastic membrane and in fetal capillaries.
Conclusions. Our study indicates that in patients wth antiretrownrc ti-catment, the pricenta
shows a reduction in immune response. Althouh we were 0i to firdvrl partclesn
the treated group, the placentas of this yroup had rantbody reactoty to yp 4 Iowra! proten.
H.Villegas Castrejon. Montes Urales 800 C.P 1000. Mexico, D F el.: 50-97-05. Fax: [phone]. Col. Lomas Virreyes.
Tu.A.20 10
IMMUNOLOGICAL AND IMMUNOGENETIC FEATURES SLOW AND FAST COURSES IN THE CLINICS OF HIV INFECTION AT CHILDREN.
Sizyakina Ludmila P. Shemshura A.B., Orlova VM. Rostovr Starte Medical University Rostov
on Don, Russia.
Objective: In some cases at HIV infected children we can observe fast progresse course
of the infectious disease, in others-slower course and relatively favourab e. his problem
remains actual at present.
Methods: In this connection we examined 75 IV infected chldren aged fro I to.1.
Depending upon the duration of the stage of gerneralzed lymph, denopathia they were
divided in to 2 groups: Ist group-the duration of the stagye of GLAP less than.5 year 2nd
group- more than 1,5 year.We studied absolute and relative amount of CD4 + aind CD8+
lymphocytes; functional activity of macrophage (expression FcR iand C3R, phagocytosis NBT
test, production of IL- Ib and TNFa), the amount of IA, M, G, (IC, aribodIes to HIV and
antibodies to the gene production gag, nef po.
Results: We ascertained, that independently upon the age (younger yroup-children aged I
5, elder group aged 6 12), fast progress of HIV infection can b'e charctenzed by the follow
ing changes: more pronounced reduction of the amount of CD4 Iymphocytes and helper
- suppressive coefficient, suppression of macrofagal link (reduction of FR expression, reduc
tion of digestive and cytochemical activity decrease of IL- Ib and T NFa p, oduction, marked
hypergammaglobulnemia of all classes), by the increase of CIC in blood serum wth the
smultaneous reduction of antibodies to HIV and antibodies to gene production nef and
increase of the amount of antibodies to gene production gag a ind po.
Conclusions: We came to conclusion, that these changes are prob biv connected with
infecting of patients by HIV stamm with high variability or with xmmunoyencti- dretermiation of patient which c auses intensity of apoptosis of rmmunoiomp etent cells
Sizyakina Ludmila, 72 Budyennovsky Apt. 17 Rostov on D[on, 3'1 018, Russia. Tel.:o 07 8632
344 150, Fax:[phone] I.
Tu.A.201 I
REVERSAL OF A NEUTRALIZATION SENSITIVE PHENOTYPE OF THE HIV-IIIB ISOLATE IN AN INFECTED LAB WORKER BUT NOT IN A CHIMPANZEE
Broersen SM*, Conley S**, Nara P', Schuitemake Hanneke'. "' -epJ. of Clincl Vo
Immunology, Central Lab. Netherlands Red Cross Blood Transfusion Servn ce rInd Lab. for
Exp. & Clin. Immunology of the University of Amsterdam,The Netherlands; ' 'Frdenck
Cancer Research and Development Center, Frederck, Maryland, USA.
Objectives: Primary but notTcell line passaged viruses ae r resistant to reutrlization by
HIV specific antibodies and soluble CD4 (sCD4). Chimpanzees worldwide have been inoc
lated with Tcell lne passaged neutralization sensitive HIV strin, he k of disease pro
gression in these animals therefore could be artificially due to an effec t hmora imune
response. However; lab workers accidentally infected with I uiitv HIVt ira nrhave
developed AIDS. Comparison of neutralization sensitivity from vus solaesfion aci
dentalliy infected lab worker and chimpanzees ma. y shed new 1hr17 ih L cpoit.n of
neutralization sensitivity of HIV I in AIDS pathoenrsis
Methods: Soluble CD4 neutralization senstivCty of viruses oeosolurn' 36'a in IV It
infected lab worker and from an 8 year HIV-I11B infected ehimpunzee wi ixacme
Results: The virus re-isolated from the chimpanzee was,tx t ',Itron too' in pi
rable to the original HIV-IIIB isolate.The or a en solated fro a-r it e), eri
after infection was also neutralization sensitive. However the v- r i coo ito
after infection showed a neutralizat on resistant phenotype which i I Jr, ix io
neutralization sensitve ayain after passage throuyh T -cell in
Conclusions: T rhee a he reutr iston r stive ph notype of HIVI BIInth
wor-ker supportsn the idea that prCiary uruses raced to be optra ar ndap~ted IoIbtrio' th e
immune response in vivo. It is unclear why the reversal of neutralizator sensto
observed in the chimpanzee.The high and contnuou neutrlizat in sen.tivty of HIV iN
chimpanzees may contribute to the absence of isease progress or r Ii,-e car!
H Schuitemaker CLB, Plesmanlaan 25, 066 CX Arcsterdamr-r,The Nethora'ds tel:+3 i 2
512 3317, fax: [phone],

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