Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Track A: Basic Science

Tu.A.200 I. - Tu.A.2006

Tu.A.200 I
IMMUNOLOGIC RISK FACTORS FOR HIV- I SEROCONVERSION AMONG HETEROSEXUALLY EXPOSED MEN
MacDonald. Kelly S,[{Malonza I, tNasro J, - Nagelkerice NJDt( Oyusis JB'.iayn J,j Plumnmer
FAt - University of Toronto,iToronto, ON, Canarda;-jUnive-sty of N oi airobi,
KenyavJ~niversity of Manitoba, Winnipeg, MB, Canada.
Objective:lo determine if HIV- I secoconveisiori among males e-s<a' e-d to 1HIV%!feterosexually, is associated with markers of immune polymorphism such t 1.iO' i scleukocyte antigens (HLA).
Methods: Mers preserntiing for treatmnent of genital ulcers to an SiTD clieic i Nairobai were
recruited into an ohs searaio cohoirt. Men were evaluated with recspert to HlV' status,
otlaer STD'ssexualbehavior; demograsphic and etfinic vaiaibies.fIalacirwiin v~. i eoliogically
-ILI-A typed arid followed foir12 mnrtfas at 4 to 6 woe inter vals in detecs lHIV ser nrtrirn A frequency distr ibutiorn of Clarus I IHA determirnantss ucia i pil'd lionm ar andomt
sample (HIV pcositive rid f lYinegative) ca thfirsome cliric poprs'.rlss an A iraiity scoare was
calculated where F =frerrcvnof erchadeterinrant srdcate d Isy ris uperscript1Ritiy
Scoie e(FA 1I_ tA2x(FtB 1iF FBIx(FCI1+ FC2)l
Results: For ty-Ifiur of 527 initially ser onegative men followed foi total of 4002- perstoss
rontf< cussesrtcd to HIV- I ser opositivity (incidence rate I I / 103 paersori rsonths.) Ceirtin
HtA deteimirnants associated with increcased and decreased risk of [fly-i,er,-,onversion in
femtales from the samire populationrtcie evaluasted.

f-fLA-A28(ssabtypes A68 & A69)
HLA-A23
HLA Rarity Score(lowest cqsartile)

IRR
0.22
1.80
0.23

C1 95%1 PyValire.46 5.3 I1.02-.9) <.0?

Conclusions: Certaira Class I MI-C determnrants appear to conifo' differeritiariscseptibility
to HIy-.Inriacdditiorn, fhe possession of a relatively rrre Claits I f3LA phenotyperis rssociatedl
sith decreased isk of HIVy- I serocorasi o nr Both of these findirigs' ciii sbcii rtr firadings
amnag prostitutes frormithre same etfhnic gi osip.This data points Ieaisurrabr raf possible
imrnsunohrologic mrechanismas wvlich rray alter susace ptibility t II -infion.
K.S. MacDonald, Mrsunt Sinai Hlospitrl, Rm. 1481, 600 University /\senucn loin -to Orrtar is,
M5rfaI0X5 fanada. tel: [phone] Fax:416-58r-8746 eresilinra' dlonalsl: nrisi~lrrsora
Tu.A.2002
INCREASED OXIDATIVE STRESS IN HIV PATIENTS REFLECTED IN PLASMA AND
LYMPHOCYTE THIOL DEPLETION
Waisle, Sharon L, Harrison MIAWinanaL, Cosarni D, Click J, eti erIlit JWells a
Immnurodeficiency flinric, The -For onto Hlospital, Faculties of M edicirnr & PharmanyUnaiver sity
of Torortsa.Tcrontonrioi caCarsada.
Objective: Icr studyIfhe ranchaisinsof low glutatlarinne (GaSH ) risreIs51ii rpatemtsvwitfh
HfIf/AlIS,
Methods: Plasrns ansd perisphmseralialod lymiphoucytes were colis srI fi sir se c ystrvc coil
trcols (n= 10). asyraplornatrr flyI positive (n=3s I) arid AIDS patents icr=20t. Frirr ieach
comnpartmentl were nmcasured thae acid soluble thiolt GSf- and 5 stes-nit '55), theiriooar
dized disulfde produicts ((t:,Sf: CYS CYS, CYS-Sr )1aad protein hounad disiltlet It.SSPr;
CYS Pr).Thiol/cdisulideirstisswerer calculated.
Results: Both acid solublle thiols FaS I.sndlCYS av in iedurcedfy 5C6 ( 1)-.(1)i tfie plisria of HIy/AID3S patents relative tos controls, with a nor insposidiry 25~0(is.0a3) iincrease in
fin oxidized product GSSG, Plasmia mixed disulfides (CYS-SC) snd plastotein hournd
dslfides (CS -S~R) were innsreased 2-3 told (p<.05). tyrephonyt CrS3 an C Yass inreduced by 30-35% relatisse to cnitrols (p<.001I) withs a nn-signisarnt. nrue n itheirs oxi
dized disulfide products. Pliunma arad lynphocyte thiol/disulfide sr'atis mess- rsdcead ira
i-fl/AIDS lay approximsately 30% (p-001I) reatsun to controls.
Conclusions: Low 1)psiasma d lymphocylic GSH ad F YS in fHlV/AILI, lapears so result
b-nm increased oxidalive stints anad tfais corisuiptiori 1 6 datacaund suppot rthiiles studins to determiane thin benefits rat cxogerrously adinistered thiols suach asc-JAr. (N--scetylcysteirse) to coambast this cosumssaptiona.
St- Walrraley.TheToronto Hospital, Genteral Divisiori, FN G- 2I9, 200 FlizalantlaShreet,
Forosnto, Ontario, Carnada, M5t3 2C4
Tu.A.2003
CHANGES IN CD4SRO+ EXPRESSION ON CD4+ CELLS DURING HIV INFECTION
IS CLOSELY RELATED TO PHA STIMULATED INTERFERON-PRODUCTION
Dllssrn F, Aladdin HassanVictor J, Getztche ftC. Skrrrhe1 P Pederseil hK. tDopar trasent of
Infectious Diseases, Rigutrospitalet. Copenahagens, Denmnark.
Objectives: An early specific loss of CD4+CD45RO I "ivirernory" cells comps~ared to
CD4+CD4Sf4A-+i"naive" colls iraHIV irfectiorshtatbheniproposedf. bowvever, the Inst of
eithter raernory r- an ive CD-4-F cells in later-taiges of diseas e as i dIherIaion ao Iaytokbne
procductiorn sin not fully urnferstood.
Methods: Flressiors of CD4SRA and CD4SRC) on CD34+ cell- weasralys'endlay flowcytomoetri on blood mnnsuclear cells (BMNCt) os 300 DIV 5- patiens I sr 102 s-sr and sex
snatched controls. Produc uin of intedferon-y (IFN-y) in 4 hioursa p15630.' 'nl'srasra nin(PH-A)
stimoulated whsole blooad was:snalyzed by FLISA i 18 patients atsd 19. sal.rls.
Results: Thaeratio (CD-4+-CD4SRA-/fD4+I 045R0-5 mas 1.3 ('392.)(nnreiihssiird quartiles) in comtrols, it mast increased to I.8 (I.I-2.5) irs ptents with a Ct 31 coost="500
(P<0.05), it was uncrhasnged 1.4 (08-2.01irs patients with a CD-' aouii nst teers 200 and
500 arid it was decreased 0.9 (05-1.4) in patients witha a CD4 moull.2 as(P' 10-6).Thn
CD4 CD4S5RA+/ I D4 +6D SRO-i- Iatmiuswis positively cornrelated 'Inthe n1.)1count
In-=0.3. P<106) sod rngalively norrelated to te age (r-n.2P0 -crptpr15 irs t rsot ins
donna nlt Thin IFN-y sufole fblesadfpaoduction mis ireased in patient It, a isa/rail) coat
p ased tsa asonts(3, I -4 nyml) (P0"10-3).Tfe IF N-y pr oduction w-ait srill -In litrar
regression positively ms m-l tel In ~thle CD4+C 63 IRI.) tubsetlPt"-01an (-o 1
nor related to far 6 D44f D4m4A suset (P<005) ill patents. I=1-I y1 ' ustioms fdd
rant correlate to ansy 1 131 cell rnatskerncosriohsls.
Conclusions: Tfss stsdy csasfirlsas a 'selective Inss olfmesniany F ' - 5 -+51' s a 51'n-IV flmiermtis Howssesverins Iater sas rtof the srsfectiona intonenaive C)t 1sac otA~ as

raeport asa incaeased PHA stimaulsted IFN-' whole blond production in HIy+ patients. Thus
althoussghsthe pooni ni CD44-CD'ISRD-- cells declines in i-elation to progression of disease.
thiis subpopulattintseemstso1 he hisghly sctive regarding IFN-y productiona.
H~asssa Aladdin, Departmnt of infectious diseases. Rigshospitalet.Tagensvej 20. 2200
Copenhasgen N.I Ic-imtarIs tn-I: (-35) 35457545. Fax: [phone]
Tu.A.2004
CLINICAL PROGRESSION OF HIV INFECTION-ROLE OF NON-MHC-RESTRICTED
CYTOTOXICITY
Bruursgaard H, Pederstern C, Kim usd fteder~senr B. Departmnt nf Infectious Diseasst
Rrgsfmntitilkt CopenshagrenDtenarsk.
Objective: To amsine hfmrsurs anse paramnetesrelsted to nor maajor fistncnmastibslsty
consplec 'MDI I nuretsicted sylrsclssc hnfrgedl witfh respect to progretsionarid duration
saf L[lYVaInfection.
Methods: a13 I lV s'rsiotive susbje'ls. with kntownatirne fer seronversion were incluided.
isa's wane 5 sampiasren wth.nFgesind sex natchted fHV sem-otegative control group.
Nat ntisIk ilsea (Hf) and-I cceIl scm)s ets 'were mmessured by flow cytometry, and NK and yinpholIte acr1vaten' killem cr11 activity mere mteasnired ira a S I Cr releate astay. Grousps were
roil pared bh a M 11W'hitneyrn. sunti test.
Results Tihetssvaor fnsdinsmgwmome tant thie Die total concentiation of CD I16+, CF356+,CD3 6 a /6D56 r ws lowse rs the grousp of DIV seropnsitive compared with DIV senonegatine subjects,'ammclow~aemriiin tsesills AID)S thtan witfanut. Furthaermnore, low percentage ard
nuasmber 01ft I clls wi-si foundmr5im thre group vwho tad had ar more rapid progression to
AIDS.
Conclusion: I 'us consmatations sa NK cells in thae blood was associated with an aggressive
ciiawoe rdia samg tthat defcive nron-aMIC restricted cyhotoxicity as associated with
3 tsLLBuu, a rnm arsvei 20, 100 Copenhagen N tDensmark. Telephone: (+45)
3lam> its is:(445) 3545/64
Tu.A.2005
CORRELATION OF THE PRESENCE OF PROTECTIVE ANTI -B32m-DERIVED PEPTIDE
ANTIBODIES WITH THE NON PROGRESSION TO AIDS
F-alta sPsicale, Lc'Cosstel Camrole, herminre Jesa-Claside. INSERM 0322.,Dntalde
Its's Ile inis ss s - s Rrirns- etm I 1aladrdt Assnimes, Parr Screetrfique et Technologaque de
Linu Bsr 813is132743Dinrsimle Ce-fex 09, Framace.
Objective: -fhe 12amaca oglobalsat)12rn)-ddrivrrd peptide R7V nimicks thre potential epitope
maca sanssar' ntn I IIV fay neutralizinmiornoclontal antibedies directed against 132m.The aim of
tis tadyi to sivestigate thteanresenmce of neutralizinig anti-R7V antibodies (Ab) an seruna of
IVY ifes ted pesostanmd to ramelate it with a nona progression to AIDS.
Methods: Sam im susamples wevefma 90 DIV-infected patents (28 asymptomatic, 24 AIDSlong-terms survivsa(0FS) lend 38 AIDS patients) and from 69 healthy blood donors. Serum
co51 r.tono ntsi R7V Ab weire detected by a tmraditional laboratory-made [LISA test.
hstadatrd mbu'a d was 81 t56 maine roaAb directed agaiest human 12w. Conrcentration of
ats P/7V Ais oexpsress as B8I 6f mAb equivalenat. Neutralizing activity of human sen-a was
folmoead Ira inil llama of syrms visa insmaton n aathse cytopathic MT4 cell line. Reverse
Ti a.-sru 5dm sc....s Isity wassolanasyed. Studenit T or U tests were used to statistics.
Results: Sm-i aIf IlV nferled pter soas displayedlhitghercnentrations of anti-R7V Ab rempsie t sd r ona''aive senra 1' tsa 2558 pg/nil (mm=90) armd 27 In 1790 pg/nil (n=a69),
re-spectsv"IsI AsAmonag ther IHIV inaected groupastir-R7V Ab were enahanced in the asymptorr.li lasto25s58 tag/rmtl) irish Ifs"_15S 59 to 1864 pg/mI) grosaps cmnpared to the proair u.aas:3 I5lo 630 pg/ml) (p=0.001I and 0.004, respectively). Among thae pogaesson
4.up sipcsieto distm ienn cording to anma-K/V Alalevel could be mmade Lbetween per sons
cluec died sortly aftes th eiIlast sampld sent tsr nun laboratory (35 to 508 pag/mI. n=23)
ric ho~se still alve bult. ick (77 ira 586 pg/nih ma 14) (p<0.03). R7V Ab level was stable
willshita m asosan asyirpams asic patients whereas it goes up anad downs in the LTS
41rouima Ini'asiogical teatofnseuitralizaine we sfhnwed thtat 17 out nf the I1B hunman sera
'pt' usdalg of equivalenstBRI G6) prevented NDK (dlade D) as well as LAV (dlade
B) HIV'info( 'is aofm)MT4 cells.
Conclusion:ieedne of sets-R7V Ab is closely asternisledmitfa non-pogessionheAIDS
and ca osaisuissamiamrkr o f atona-ro041ession.
PC -I1O1CI-6 Unite 322, B.P. 33. 13273 Ma'seille FCddex 09, Francre. Telephone: (33) 91
82 15 10 -Faiv(3t) C141 92 50
Tu.A.2006
CD4+ T CELLS MOBILIZATION DURING ANTIRETROVIRAL TREATMENT: INTEREST
FOR EARLY CELL ACTIVATION PARAMETERS
Amissr B.I 6 1,sb. laaramaisaloe. F tRS0625. 2V'mologie. 3Mal. Infectp..Pitirl-Sallatris/ie.
Pas racems

\O
0
u
cc
0
a
cc
cc
0
cc
cc
266