Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Mo.A.51 I - Mo.A.521

Monday, July 8, 1996

Materials and Methods: 1 95 er from'1L different Afrcanoures were tested
(Senegal,. N iTop,Bukr FasoNiger N co'r aT Faid, Fameroon, Gabsn, Coego, Bar undi,
7amred) Thrrsera eteN ollect10d amrrgAIDS ptieers, tuberculoss patients, pregnant
womren,dIlod dnr'cpo)it ir rrand t D rtrert All te sera were tested)forgroupO0
antrlrodies witFrInrrnprr ri F[LISA twirromrb r inaton oFV3 peptrde fr ore ANT-70 ted
MVP-51 80 (Rerrch r ii t r rr' iot~i c Belgrim). Sera rerctive by Fli r wcrc retested in
a neoe rrur r.iy/(LA).rnwhcdiafferrntr I tir ated V3 peptides (corsersuci HALANT
/0,VI686, MVP-S18F) werer'appliedas tr epi ivi n rormplex in parallel lnetserr rylroestrips
(Researrcl produC cr rprrr'cic, Pr ru) amSnpes rerctrve re E isa were also retested o
aniF eluse westerrndbloI for e rr or cr' i rrbode te p12Oof HIV- Irrt 70 Sera were
coderedlar ps tin r otIll yrop ()rnibodic' rfthey reirted or LIA ecluvely wth
grouri0rpFptiest rwhether notrhr erasreactrionror the ANT7 WB, or when rntrbodesto yrouDOa)ir7nd Mpeplri(res were revereIby IA ircompaied Fy reactivtyitl
pp 120 rn ANT /0 WB rmplsr reativr wiFh gro101 0 red M peptdeswtot reactronas
gp 12F0 rnANF[70 A"Bwrrr( roniredasirdeeriteelor FIV groipO rtbode.
Results: Arro'0 lhe 1 085orr tostodI 5513 wore HIV posrtivc, 5881 were HIV negatee
ird 27F were HIVindererrmrinatFir n hIttiairamorrt f serr tosted, 17 were positieeFor
HIV Igrorpfr F)ibIdits ird 5were rnleterminrate.
Sera consideredIar posyie Ifror rut()wr r ilpreioslyidertrfied is being HIV poriter
and werelfoiln rdrin fteIAfricrn atro 'stested (Seregil(), Fogo (1, Nrger (IL,
TrIa i(d)r Faior (2)FCireronr(7) rnINigeriar(I)).Among tde 52 grop C) irdeterreir
Ic e er-r5I were F)IV Iositir Id Ni DsIV rndeterineate atrrhley weretiertifed in all
the 12 coirntres irncldinisFr tldr
Conclusions: The re ivienceofI IV o sp O)vroses svery lrw (0,3 iamrig HIV- I p
trveeracii t do t resic tellto iiareronirlnighbouairy eotroieslke Gaborrird
igierind erritalsbelurdrlrWsiAfrrca

importit tdrtpatiert risk irdciirarinformation Ic piovi'ed to nre ro iiiuchi pe
cers are reFlexedto supperrentairretFhodrsinlcretin'tIhr'to lcig l ti

Rchard A. Cr -uHIVLaboruV'try, i Mi ntry oF lix
OrstarioCarada, 19P 31TI Tel.: '01) l235 571.Fix: '4

Baid, I Ftir'e

Dr S. S ueyerrr'NF rirbiSrvicr Ir Brtrir
Dikar Serrepair16to:21 )12 (21 F x) 212

ie Hoptlbe Dsrtor BPP/73IP

1 '12

Mo.A.51 II
HIV- IM DIVERSITY ANALYSED BY SEROLOGICAL SUBTYPING:WHAT ASSAY,WHAT
ANTIGENS, SIGNIFICANCE?
Birir Frirr 'Fltier] C. Buziz L,lPossn 'Simon F' etters N*'U'irer t F
Baer')s, orsFrnce, I I ph lB F,uisi rirce DBTOSFFOM,Mnrllier,tFrrree.
Objective: T o vopiprirft n' ssais oIiroicpilisubtypngof IIIV tt I s cir)an
determie their- vale orepirer i~',i,stuso fHIV- I Mclverity
Methods:VWer, trlIzc I3I isi C'Frtideso rrpreer lrg terc orenssisiqe lI F e
V3 regrons from )8 1140 pesreinrdIly rrretirrnaysiD(yer et lr,FHunin irBe-r erruies
ard AIDS I 194), and I 1 Iubypir direI by pheretrc inrlysis(Kidbertet i, J Vrr19)4,
68:o730).Bril itdIrecI irdislbtye-speif iud phre blockngerzyme rsrrirarsays
(EIAs) were dbriedc i 7 1 ar i'd Ltd uiir ee eoligopept des. More thanrr1I0FOFerr
collece rlr IirHIV N IoMinete rol K Ialin r '1nsintrerwere aralysed usirgtese iasys.
Results: Idiect FIA wone v~it i 'll iorsrologpcal dicrimrratiorwhererslquid lharse
dlecking IAt 991 IA r 'allweo d11efoine iarl a erolo gcrsuorype rinpprocrritry 9C
olf te iamples.Bolb ryi ii r 1c r ly F sexuil pirtnersiridanysi Fyger
grappicil Ii trili n Iofevi' ilslbypsdrirnsraIteed eFghrelabilylF e
SSEIAs.Fomprirso lbheSSIAs roulswiFh 'eot01p10detereminlrtrrn i ppixitly
200 indviduurs sIwedlh yI i vilur'oI the SSFIAsiprod irg te'orotypt'oflIhe
irnFcrting srun,pariculairly Ior-l t B, 13, 1 rod FIsubtypes. MH e r51ir rr n ii 'ranalysi
iniated Itic'Ireusxiner Ic 'wro )ior nedrP~rxxx oIV3 ire lhe misirdetorrir
HntslfSr subyp"specificiy
Conclusion: WeS'evoped'EIA; 'otia;y' 4'ri I, crsteffectett.-cient abrypiy of HIV M
infecto. I hreeIoos shuld re very eflfor lroiddopiden oogcs survey ofdeerity
particulirlylfor- doprngrneter iir of' c rsrrsitirollf uttirevacintes
F Brinr, lid r ii ro deri r or' DI) CH iirio. neu,3/Fl) Tours Cedex. Frarce.
Tel. (33 '747/747/ext. 11086Fax.ii 3 t/'77310
Mo.A.512
DETECTION OF INCIDENT HIV INFECTION USING A REFLEX ALGORITHM OF
LABORATORY TESTING METHODS
FGalliRihrdrA. Hijr CF Frir r H. swe r'C. Frnis AGregory B.DIV Labsiatorp
Public DraItF I ii iii riy Sries,' OntaiioHinistry ofFHealdh, For onto, Canaida
Objective: To txaietreoficayoF ofIi iForator y tetong lrgorithsm For-detection ofindent rise oFfHIiV ir.poltioniiswIxith airynexposure cseopor ies, wilb partircuar focuson
the wndow pFiod oI Frecrio
Methods: A cier' ortireals is oillheresu0lts If256,265specimnrthIFat were sppliod to
aI idrtrstiy rip t i illfrIFiV I'stipg roil hd wis undertIrkon for I )95.Tdo algoridem
cosid of'rniit'iltio F/IViu ntio'' scre'n''ingEA asweol,cofimatory Western'
Bot WB iuppleetar aiibiii y aisiyisFrhisIiV--2 WB and arsecond IA. red errus
detectiioismeiodsIsicI r p2,A,'.,IsNA P B iinI rlirire. Spocimers ire rotoexed throrigh
differert aveiesiir' thlit'indeii dis ing'on resIre sts indior xoxirurecatsiory ird
clinricaI l i alion r viii deId. Spei ii c' Iiror r r'p5c 51eteIrs r it' kreFdIin r i priter zod
ditadiac' proviedcip lxsi Icr xidetyc I:tablilied
Results: A toialIof dl5urnat ntr. B 8 elesi,/ sex urlrown dot rtio
serororrersi r I DIV ir 1)'. cri iii' toIc1 10 malos,1idler~s e seox urkrown) in
199a. f the 15'incident cissSi9,/ weriertifoid iiiisaresutcf a osite
p24ApgItesI:39/ won'eIFII7 iitibys or i prtice Ibitwere or'led Icr-p24tAg testing
becauseofI oxpcsare catloy rni aIi i r Ifomionsan.d 8/7were.inti body indeterrinate OF the 95oigr in egiroc.spcmer, 4 retct'd postie ur inanewerilhripger
erition i' recminrrirI IA (Alil II A ol ifI1 o hee1/ patronts wore FirstItIme t'ters
it the tioeofp2'4Ap Ietcion, ar. Ii 1 7were sor positIeo For atibodie uo rlsw-up
testirg 2-B wooFs laor.No IoIlw-uili titen lad bdeo rceieed For tde renraining S
patertsbytheenord 1 995.
Conclusions: A c orpete'labratory lgoritFF0ircorporating ippropriate carefully selected
antibody screrrand sppleenta Iiing mr nethods is we)las eirius detection tecdhniues can
identifyincidont iar's F HFi rage pecinc that nirbrody testirg alone maymiss. It is

Mo.A.513
MULTICENTRE EVALUATION OF ALTERNATIVE METHODS FOR
CD4 LYMPHOCYTE DETERMINATION.
Ly inya, F.F.. Scdecdter H.,FrIcheer r ide Pciroz, iVert iii 'ro. i F 'r MedHoa
Centre, Di es Salam); (FIsphitaFUIer it riBi iz.I, Jnir cit Ii Ftl 5cr ox or
UNAIDS, Switzetland)
Objective: Tsoealote thopod'ormircesIof or smpotr-iaidcloilpOr ltorinati thor
for CD4 lymrpFoayplcdetermrriinrtionasc irrnred Ito erefr~erce'misod, 'fIow c' ii o
try) ir lossinuottiaized conrcs Brz frniu.IPrrozr'a)
Methods:The per)fraceofltheFACScounti(BetornF) I(-ini - r ix CF)'(Ce
Diagrostcs)Dynabo i TI T8 Quaitive yreriodlDyr l F Culter Cvi FF00r
(Coulter) wore cmpir'ed Iito oeo(Pfow cytoe Oty chcFoutry ova ~i on' cri nr
methoird ilerolled i a icier ol50HIVrnepive ire "5HIV p osiie irdivduars.Putnts
wer tIrifibeditlreeogrops -)0,2005)0,'->50Fsoate LDI yrrl dCc ounts
Results: Allme'hds werery toperform oriwever'Fe - rlc i oririt h
Dyriberdsmois sabdrious id to soeextent sabctleerasisthe vsual roiip I
the o uter Cyt spderoTle nurbe'r ol tfO riin-pe h t l relprfrled equte', I
daily bais is lInited to approxiFrairly) 0TRAx-FCF);:2),FACScoin'; i ot
Cyos pherrS5,tDyrilicudsdhe overil coirelain fit cienFl cr1 aoned cio frent cuties
widh Plw pcy rery were: r=096-0.7,FACScont, r =Otis 0.9i, Dy5c s r0.88
Cptosphere: nre=01.84-0.8), FRAx-CD4.inIcgereral, thr Ibsetvecorrelains wire psligry
higerlfor ther DIV posiivoptFestot liiieIF othFe HIV repativeor' o
Conclusions: Ic thre twocouireswherxethey wetre 0ialuatid, 10epeorncir o'tne
FACSconh rib the Flyr It ilsit4F8 QFlitivuemethod coireatied ccsI witF I~t F thFie
Plo w yinetryF Dsrs dart rsuliswcre on o I Fr tie TPf DzI L ISA TI rii 1 ticrite)
to intertesthkit prornan c'.Thc' rresuits lcw tdrt scira implr andi eapeInletherr it e
CD4 etodolo pe s y br' i v.ri7 p1tinIorCD4 in cleia stira;with nimtedfiitiho
Dr. lyre iyr,.FF., Deprtmn't ofd I Lc iolyiar'r myMHoistHot 'Ficl r,
PC) Box 6'218, Dirr'r e a~,it.' r I Fix: u255 c5'1 5)1
Mo.A.520
HIV- I SECRETED VIRAL ANTIGENS(SVA), POLYPHOSPHAZENE ADJUVANT
AND SHIV CHALLENGE MODEL.
YcdenLu.NealTouzlirrNichFl isKilter;, Fr tc CbuS kwIki Hrr y.QirIShre
Jenaks, London PayneBryan P oll s.Virus RBear'hIni ie, 6IF)oiton Stt
CimbidbgeHA.,LISA 02 1)
Objective:FToealute thi'eoFFcacy For vlIII vri c fr1 is iiiitncop oed oF e rot
eb vi iraatipens posrunique adlutix ltn'osmokey,,
Methods: I) DIV ISVA ire prpardurobmPrin i d st nc cilith iii Iasfrm'c'with
geroticaly'egroerobdHIV- Ip o itht isrblot o xpt.'al nlr ii is'.pr'cir u
boo'sriotcotin FDNA sequncet'hiitiFreentia ot irus i ti it ioIF-ori slyes I
DIV- SVAhaveeenor idnaeb, FinldngtrveopepotIns rdot omaisit it oFE
subtypaoCH)5, a primsary iet o tIcflB rtypeb89F.1 Iiorti srii n D>B.
andI in ergineereb envelope protein i whicF tIreeoe f tilv viiablelops1hrye Iberr
reeoseb(inccllaboration with JSr'rr Di o HrvardO;'2) 'oypb spnizcc'' i wildr oLu
syrtdetic polymer s used toenobancole she i n got f 1110 1]V SVA: isn1 3) Irnrd
tier to tetieg antibsody ib cll-noad omrounenrsp oes i ct'disoke'v SHIe
vrusprepareIb-i nofectedonkey F BHC s sd cit I neotpii ii ito asto t
ti' effcacy cI the vacinto
Results: The immunegern Ity of DIV- I SVAiscorioariblect IFt eicited by wholeIinaltvi'ed DIV n ireraibbits and monkeys.FurthIermiirto DIS'SVA cint inr r eariable0loo
botheroninitie orveo pe hFr Fentetod ho e oadjuviatrIFectIof p aypb iF 701 oseinifi
cartinr bothsrtrmsculrsn intrir ial rncor Lit IiFn.SIV hr benoon e ohllnpe
FHIV I eaccinrted mnsrkeys. Antibody acivty ell-ediatvimirner o r I c it I proto
tion agairstISFIVecihrlengrn'rhn~esasImonkeys xcine' 5' wirhtihif Dt IV1SVA
by different routes will be sumsmarizedi
Conclusions: The pinel of genetically engrnecird DIV- I S/A it oep otntasi vorcor
than an envelope stuort errcrae. Hosticrpor taFly. DIV-'ISVA i ion ro trindItoprburr thin whole inict ileSDHIV- ITe c iti rofFHIVVWA witn oypisp1 iene a
the ibiuvint has potential oIbecoritgpnow FIiVIvacineci'nd ite o Fr Imin in in
Fur theremore, SHIV charlenge iin monkeys mnkFrit posilet isctltivt IthrelFf icyof trio
DIV- e arcirecardibiteo as tocverc oerseolitedbresnur'e tt' I nipirz od lr.
Yichens LoVi us BeserrcF IcsIitute,.F1HMciton Strct, F.n rei to."'A U.in3, IA.
Telephone: 617-86)-h232 Fix 61/861-Fill err i 72 ) / ii ioas i c i
Mo.A.52 I
IMMUNOGENICITY OF MODIFIED, RECOMBINANT HIV- I VIRUS-LIKE PARTICLES IN
RHESUS MONKEYS
Raf Wagner-.Ver a Teeuw cc2. Ludwig De (,onatFaso Ieoocsz, FlnsWol'. 1I r 'eof
MedricalHiroraiolspy Driversity rf RopenrgupFoe n 21TN >Pr is tceto'01r, Ilswi

I5