Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Mo.A.401 - Mo.A.405

Monday, July 8, 1996

virus wchv trhe raire ofI0- t0"Vrirlods ir vainactedP"criiasriene 'tie red
by limiting drrtion ci PPM( andcIirlivtrir rwith C8 i66 cll ircmularing C.I irre cror
free vacc rinsw(e re i id rlrrrusingSV innectdat iv 'ira PHA-1-lats. fit
weekseatier primri rirfe tr with tier riirtvirusalrrmacqueswere ( Iclls.11nta
venoudy withi30OT(I -loftirvirulent, cel-reed tiofibiVir r, derivedifr n i0'pierno
arnicique infectrdiwi tice iVrrrcJ' rrrle clarrn. PCP wacedti I a,r "vac
cc''e" nd chtlingyr rris r rier riperiiin ( t r t c allengie.
Results: Tworweenksafter iroculit in withr tternuatd\irustte 2 tpiirciiif r r cei t hid
reced the ihigirest dices i)04in)d0'MI t iiha ryrirni ipl)lwer i rsoidstrini
trose thatinreci wrie r dos f.ly 6ver' pet ichlleevr r iads were siiin bohi
groupsi Airer ygeneredSIV-speif ic int iesirpective ordrse.Three ort9 ranrmaas
teted hid net-speciftic en es aftier pimayi ncuiror, t itiere:were unrdetectbe ib ty titimen'fichalinny Sniper ii e t onr enistrnce war'snen niyci iin ireaii th at
iad beer intected with inti d oes fttiernuatent virusc2 aiiersn errpletelypreinte in l
ienge, rid inia tin 1 ii i rnvi rswi 'ine rhctaeby PEciripnct week- finr chalrnige.
Aiieother aimalsi, riniti i r nice ciitrlin reireiecntedwithte challengvi i.three
f 3 animerrs inthi h otet ncr -ip i lideprotic ite Cliacirity toSIVnt iiierchillnie
comcpared tin0oC ttic in theilow ccst yrou.Nco['P relt ioniwai errntetweenrthn
ecagnitecite aiii SIienvelope aticontarenpocne icear ned ci tire tryrfchatlenie
rd reristnce' tostier ii(tioci
Conclusions: Hirgh doss cf trtenuiited Vii n iiarereqired to indutirlyi p perninnirci
reitninrhresu r r~ e tirnamrici oiviruc ippecein rierun iooP
oeenrcearerr lniwi iiaitecidwith tigytose n t itentedvircs.Frer llnipe icc cci
respen.es esred inriit c inrinntwithiipinoeinowever,onin ii iii riven high
dose o critenni r i s rei.ni ef ci c iif (t1.rennponsen detncted ifteniciaienry. tiken
toethrer the rinultscucii' itit seific ir iiinnatiirecnnsesiht ciunricis'n iiarnrpicrtier
ef attenuacdcind aruin in'r triticlfr rier in' ti inricerny
SA.Strice, F AiiP PitriDoicr, Saiiisbty, iip tsa.iiSP4 0JC, Urited K iregPom
Telephone:4ii19hi P125ti't ia it i9)i PH P3ft
Mo.A.40 I
IMMUNE PARAMETERS AMONG HIGHLY EXPOSED, PERSISTENTLY SERONEGATIVE
(HEPS) THAI WOMEN
Rune nne ciikit it c for iprerr A',S iyanniV1, Pioneers H.',Nryrct r iT *,
Tirntri jA,. Krirn ', de P. ii i'P'''Nelscc KM '. higMai Uive rsiny
Chceng Mai,11in, "'CD)Ant ir, Grc USA, ''O(NRA[P Atirnirta,_A iUSAi'Jchcn
HopkinsnHrity, BPlirei, MicitSA
Objective.Tc rveiantpn' r nuon iiniaameers inscind withiackiof exualtrnmirion of HIV to wcomiernin'spine r'peatiecicprsare to i ngle HIVinectdP(IiV fP)painner.
Methods Hiphlayrexponr'd persiictenty scmiegativeon(HEPS) wower were 'rnected frem an
ongoing ntudy oP IiV irrii ioiiromiIii'fected tHuIV+) rmace biood donrsinotheirr
wiven (N=50Supeinn t irpi r'aposin' win teficed tby iiprotecitednexcuai iciaity winh a
documentied HIV-+ arerr frigrieatperi thii iwoyeaires(cidi in dcuirentenx p ionuren: 3.2
yearn,;rrrnge 2. 1to 4.I yr ) ['Poie, crPdin rser, who were irire, were ear oded. Imnree
panaenern ci '4I t PP riipaerrswerencniparind tihone oP59 ierngaivaeccwmee
wih nhror t mcirci or- id'termiiinetxoire, the sohsci oh 22 cmeregativ arpicipanrtn with
nhori ternm (<'yenrs)ci iii' iiand pcblished reherer eThi i cpouton (N=-210.NK
reins weriniireldtbp i ii i ctiri typi ' uinripLeo-4, Leo- i in rd Lec-cpp wornonionaatirhodes isCD 3, ( Ii) Cl ))PH
Results: HuS partiic irent; It( it difer fromiioher eronegpive anriipaiiisccihr espctn
tVIP con, ora iii p yr ii cii,,t Pt (Ph) ioi % C (8%,tP't c nior
CPP8 caunt. PHowevier, thery thadihigrcimean'iNP celiper cerct (24.5 %) thniiter thtie
cironregat a ivecsty womiinawith ht itr minate inxii'unap ccc8.4~!).0 i ), throne
with nhociititera poe cr' 0P%, tP01, or tin infercincPppuatticn ii9 'o- ieailK
celun act nogIitfP ~ i ~i iiir'P)waigiheir tnt armicni pronaice sinn
women with hctrt or intrmirinaic apore ('9, P-0.04),tosn with I ri t iirn napenone ('P7, P=02 I) r iii rcferre in ppuion (45 't2).
Contlusions: tire n'preiirr yripbservairnsirisete posstibiittrntrkillk '-61e pupy
a rote inci resin t IV ir io r.rc cwcmerwith repcaee np arc to r, rng
HIV+ pirner:

Mo.A.403
SUPERINFECTION OF HIV-2-INFECTED PIGTAIL MACAQUES USING TWO DISTINCT ISOLATES: IDENTIFICATION OF AWINDOW PERIOD FOR SUSCEPTIBILITY
Ottec Roe A, EP nit' er Pt[)-Adamse [R, Fr Pound CA, Jancksn P.S torheire n C.Rayfeto
MA. HIV LaPor.taryci r iratronre Branch, DASILK, NCIF'. CDC, Atlrn, CGnorgi
Objective: Te er r ilsh nn;tidy tromotypinccmira nfrecticcc wih two phercotypicitly arid
genoiypinallyaddstirctipr inmarya isolaten of HIV-2 uang an etib nih ed run ialcmdcineg nynterm
for human reminoan fection.
Methods: Chnallenge tockn (--02 macaqueonfcti one dones)forar hipt nyr i a-foringre
nolate fhoerGouiBinnau (HIV-2GB3I122 aind a highly cytotyt n ioate"romCioeind iv n
(HV2CDC6p8) nonsisting of elfected cenla and supeircatanet weie generarind ty piopagation
en a heterologous macaquececciipoet hroniconsiterntdononin. Crop ci iwn o to ucimeals, preciously infected wth eithen HIV-2GBi122 or HiV 2(cDCh 8' were naposed to the
otter strain at 2A84 8. 12, 14, or 72 wks after primaryp cocuatroe.Two ie'n rl were alno
nimultaneounly eaposed tnebth iP ruoprepari ons.Alt mcaqucesweremenitoreP iti)4 wk
iciervalsn for chalenrge cviron by rsecrsitrve, iselate-npecific pi air ii PCR PoPetii iasrayu
welt an isunarcultuirring rcnthodologies.
Results: [uat retrovia i nfectiorn could tbe cstablisehed by nrciutianeouintciiueino
both heterologoos felddnoltmen. Soper infection wan nine nennible,,if via iihalenge omar red
at an catltimneciciervaliiafher primaicy infection, hot before a fall nero onvers n cV i i ca
leniges at 8, 12, 14, or- 72 wine pent incunarime with the init iirnfectin g strain failed Io icsuli
in an cvcii mixed inoetonultsnof neck infectionwith anticvrindmncaqueinl ndicadt
ed that pion eaposure in alltnypic netl omponents did iit prevent infectiin
Conclusions: A window period forsucnetptibiltipto naper intenteion pycvxit tchaoud
ive riponrttimplictirons ion the efficacp of retinalevacinn, especipy fr 'I aid iul
who ire it ccirp high rink for exponuren to muttipie viral sirainCdurrenalts' r r rac,=esnt tat
protection from huarietircnnrit ifertin c pesble, hut me ip requinn frt i nteraci tor
with a replicatire entity. Additional ntudren usng tern nodel shorid far tier errhin ca or
anderntanding of h ithe cheervcent enceptitbitrip to end proinction firnt apen n ferin,
Ron A. Omten, Ph.D. NanStep GC IS, CDC, 1600 Clifton Rd. Arlat caA(0A33PPUSA
Telephone: (404)639-t10I8: Faa: (404)P6P9-01I0: email: rant umn dd i.n cc ft
Mo.A.404
IMMUNE CORRELATES OF PROTECTION IN HIV VACCINE EFFICACY TRIALS IN
NONHUMAN PRIMATES
Heece onithain L*, Bogcr, VsA MN. Nrc P' FleauweriV'.Pacipatinin' scmiio
the European Cenrei ied Faiity ior pier ri i alHIV vccine dcer-rpitcmi K r
ircedicat Primatc ReseaiiicinCentre. Pit wijk,Thc NetherIands
Objectives: To deteiraine the cmiiurn Pf HIV I anccine iducad mcci unerneinnsewhic
correlate with protection firotiooocnusin well as tent 'rop ou anlhlemnye ciihdftnnent
isolates of HtV- I iniccmepazes nmd St IV in rut mon kean in"ontivcely
Methods: Within the PCi -F programnme i comeparison of rnprrune responscc was ridertliken
ic flveedifferent HIV-I nficeystudies perfonrcme n cimpanzecc andor mice a rim'nkeys with
PHIV I caccine candidates. Immune responsenmenitoned irecluded ccirnmeditned ucep e:T
helper (Th)(ecuneertiroe of antigen specificIfiNy IL-2. and IPP4 eceting cells, ye ch cpu iicraice aned rytotoarn Tlypetocyte (Ct) r esnsruesto ciiis peificp epidPcsindI n umor ii
responses: eiruanneot'aiscauion tmure ad unigem oeptide speifc antibody tinrn-n
Results: Protectio rorierein fectin was ohncrvand wirh tww Putterecciscu n iHIV- aciner
candidates in e cipanzees aied cccsutnt HIV- Icancnrc' candidaurnoP rhre ealinarid for
efficacy in rthesus mrocnceys challenged wih heredoneoseoPeter eogo i I iIVl envaelope
containing SHIV. In ech cane protected aniemits had the hihestiThe tem (IL 2 is elansiILPresponses as well in neutralnsing antibody 'tee primstathert omoogous slate.n TI
nesponnes were canrible hot detectable in rhimcpanzecs In r hesnurmonkeys TI mcsponsen
warn obserced mteconsisently to Pifferenet peptide psicnc c mc d niPminurewrch
were protected from SHIV infection.
Conclusions: In cases in wtich arrnimals were protected. n conrelarimnroP the au i i f he T
icelpen immune responses in associationc w i ceutr airsireg urnbodie cac i n. Neithera
Fh type I census type 2 response nor the presence or absence oPC TL mespenses was
found to correlate with success on failure of r vaccine candtudatc to clinit proeivnnincmmacnty
Dr. J.L. Heeney BPRC, Lange KeIcweg 157, a2288i C)RP1swiThe Netnerlanns
telephone: +P3I-15-28126681Fax: +31-'2843986 P-muml: ter p pnml
Mo.A.405
ANTAGONISM OF VACCINE-INDUCED HIV-SPECIFIC CD4+ T CELLS BY PRIMARY
HIV INFECTION.
KenStepherrICreci berg P Hoffmane M, Corey I, Me~licth Mi. Pr nn 'p Vshmington
Seattle, WAHSA.
Objective: Definng imemune responses to DIV- e arcnieecwhimcvead c ' cb r avorable outcomescuon ttIV- I exposure wilaultaiumatelyimoenp ccHIV vcrcieP' C n
Methods: T helper (Th) net responsesnt HtIV- Iwere cioned med inalpznd min m eipient cf

Krimp n ny m mrem pi mi r li,Rn'
PC. Bra 80 C(NU,<tuirn' Pi

Sii mii Him:lli ScencesCr nig i 'PnnUnivsiy,
mm tn 1;inn' ur 5 22 1966;: 6(-Ci 2cv1'tiP

Mo.A.402
ARE HIGH-RISK HIV-SERONEGATIVE ZAMBIAN WOMEN RESISTANT OR JUST LUCKY?
Knewini etoin F, AlIcrn5'."Vnerei in mc 'n. S cliii u' IC ran PB, Cnsn tr n PtSlneppaimP II
Californa Dept. of Helth ices,eaen. l e'.. andPU51 '.Sum Fmrmmcisco,.CA
Objective:ITodeurrnne' it c'cul epon'eip m Ic romeinrer-nrri. 'mmlioifih-r nk
DIV emrng ine (tIV-) Cmi (,i( -a.r''en.
Methods: BeePd inomncrur iiirighsu 'inu mared i t iV1 irene)rndlenrik tIlVCatch mc women were n m an'zeI no' in pru e nripine'ra blod lynnpyteyinPPI_)proentera
tree responses to C andi in I mm ntyrn iorPHAlcanlscifCDPT or CD8't PBL, 1L2 pmrodor
ton by PBL in responeatin I IV cmen'ecure peptiPen, pinanmneels PfRAN PES, NIP I (a or
NIP IP fanrd(D8 "nnppr" t,c I l"
Results: POi l the cnn u,srne'sutid nty thupricerinnnie espomcsescit P to in ci'cin
ular lcger en Iinn r l i limu' Ii' 2 1were' pig mmcatip dferent between Inupisrenaand
townrink womnc. In timer'mminitemd eunep ernietthe' responsesto one' llogene r stimulatoer lne mn(IBC)21) uecccin pificnuntylcwer mm PBL inrmh n nrik women.
Conclusions:(Oucir esuai mnstiirute itl ect icelyplowen prolier amienepones to
soeeealt anrtnycncsl miitentther mmcc nne rn-n ' dciPuin'gnnth 'ih rme nd Pow-rirk
Cietni;bawcrrnr (nmm isdftir nce maim mo'In mcrienm or caposinntonabinfrcnmli ' mou nts an upo r t I ofl IV fn _ 1r, i ur -s rn I 'ihl o it' m a y be
respoibnle fcr esm 'ist nt IV t~l ii' I mSnqnnun~ion PftHIV/IB( _21 nrrenreaetnceT
remus mm lymrphndemm P c m or min ioi i nhe hnpa'ipheralibPood 'ea be c"esponsible
for te selct icely lower pri fer atia've usmcnen Pf PBI of the''high-ink'' donors to IBC2 I
uniyens.Strien nun in ~ pugens toirunt iyimmunologic aland/or genetic nmecharnisms which
reap prevectrinctinm.
John Krcwk,2<N ayloPrm S.. cnrncsurr rCAPP1 2 Telephocc: 5S084P-526' Fa:5 10 -'PC 2'79, turn 'I l )h tilt1nrA 1I u Pt1

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