Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Tuesday, July 9, 1996

Tu.A.370 - Tu.A.374

Conclusions: This study allows us to demonstrate that despite the Ireen -e of the HLA-AII
restricting molecule, not all HLA-All HIV positive individuals are able to e! it s ccific
response against HLA AII restricted epitopes.
B. Culmann -Penciolelli, U445, 27 rue du Faubourg St Jacques, 7501'1 PARIS, FRPANCE.
Iel: 3 I 4 07 18 21
Tu.A.370
DIFFERENTIAL GROWTH OF HIV-I SUBTYPES IN LANGERHANS' CELLS. RELATION
TO TRANSMISSION ROUTE.
Soto-Ramirez Luis E., Renlifo B., Marlink R., McLane M.F., Essex M.I H,:.rvard School of Public
Health, Hasrvard AIDS Institute, Boston, MA, USA.
HIV I subtype B predominates in the AIDS cases of the US and Europe i it fe-sns transmitted through homosexual contact or intravenous drug inection. convrscily he eterosexual AIDS epidemics of Af -ica and Asia are associated with no -If.btyiles. HIVI infection with subtype in Thailand and C in India have rapidly expand ed nrd overrore the initial B epidemics. For heterosexual contact, Langerhans' cells (LC) have beeon proposed as a
pos sible primary target for dHIV I infection. Our objective was to, -r- - the rblity of
HIV- I isolatstes from different subtypes to grow in LC.
Methods: Epidermal LC extracted from healthy human skin through a dis continuous ificollmetrizoate density gradient were challenged with 9 subtype B pr rr iry 'mrrates from US
homosexual men, and 18 primary isolates from Asian heterosexuatly Infected people, 16
subtype E from Thailand, and 2 subtype C from India.Virus' repliration was assessed in LC
and control peripheral blood mononuclear cels(PBMC) by deterinir,tior cfr p.1 levels in
the culture supernatant on days 7,14, and 2tI.
Results: HIV-I replication in epidermal suspensions with 90% av, 5 ]s-fc I C: was statistically
higher at all tested times (p 0.001 U-Mann Whitney) for Asian viruses, with rul differences
between Thai and Indian isolates. Contrasting, all isolates replicated efficiently in PBMC, with
no significant differences seen between US and Asian isolates. No (cytophatirc effect was
seen in LC cultures. LC tropism was independent of macrophage tropism t id of MT-2 cell
growth and syncitia formation.
Conclusions: I. HIV-I subtype E and C isolates replicate in LC,at ignif antly higher levels
than does subtype B viruses. 2.- This distinctive LC tropism could explain the differences in
frequency of heterosexual transmission of different subtypes of HIV-Il aed the major differences seen in the epidemics of Asia and Africa as opposed to those of the I S and Europe.
3.- Tlhese findings support a possible role of LC as a primary target for heterosexual infection. 4.- Vaccines intended for use in Asia or Africa should be designed to account for these
differences.
Luis E. Soto- Ramirez. Department of Cancer Biology Harvard School of Public Health.
665 Huntington Ave. Bldg. I, Room 903. Boston, MA 021 15, USA Phone: [phone].
Fax: [phone].
Tu.A.37 I
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF HIV-I GROUP O STRAINS
SSaragti, I. Loussert-Ajaka*, P Mauclere[filled triangle], D. Descamlps, O. Bouchaud*, F.
Simon, F. BrunVezinet*. ICGM, Paris, France, *Laboratory ofVir ology Hcipital Claude
Bernard, Paris, France and [filled triangle]Centre PasteurYaound, tC amnoon.
Objective:To char acterize the genotype oand the phenotype of rItp i v-usias isolated
Lrm patients living in France and in Cameroon.
Methods: Strains isolated by coculture on PBMC were studied. EDNA was amp orrified using
grouip 0 specific pr mers for the gag and C2V3 env regions and e-ecd.ed (Applied 373A
sequencer). Phylogenetic trees were inferred using PHYLIP pactsre SEQBOCT DNADIST,
NEIGHBOR JOINING or FIFCH, and CONSENS). Phenotypic isitsitt to nucleosides and
non nucleosides reverse transcriptase inhibitors were determinedusdI a ri andaridized in
vitro test which esluates RT inhibtion, considering the replicaton k inetics o cr strain.
Syncytia formation onto MT2 cells were studied using standard technics and correlation
with the V3 loop amino acid sequence was assessed.
Antigenic consequences of the strain variability was studied by the sero logi cal rectivity
against ANT70V3 peptides.
Results: As already described, the inter M and 0 genetic distances clear iy defned two different HIV- I groups forming a double star phylogenetic pattern Inter M acond 0 groups
genetic distnces ranged from 39 to 49% for the C2V3 env region nd fain 24 to 32% for
the gug region (our work, J.Virol, 1995, 69: 5640). iHowever, the ana sis der siedl foera the
new of group 0 strains sequences suggests that the inter M and (_ oenetic 0Cstances may
be closer than previously published. Ehis variability is correlated with to- I hi- pattern of
reactiwity against ANT70V3 peptide.
Conclusions:The emergence of highly divergent viruses must be talen ini account in HIV
diagnosis, in vaccine and in drug development. Surveillance of newly recognized t IV-I group
C viruses is important in order to monitor the incidence of these virus s nd the emergence of unknown viruses strains.The use of a serological algorithm for the detection of HIVI group 0 viruses and other variants (Mauclere et al, in preparaton) was possible after the
setting up of a surveillance network.This approach which allowed us ahready to characterize
I8 group O viruses in France, confirms the need for an extensive epidemiolgical study.
Sentob SARAGOSTI,.C.G.M, Institut Fournierr 25 Bd St Jacque-, 70 i-, Pris Frtnce
Tel: 33- I -40.78.26.45. Fax: 33-I45.89.74.05, email: [email protected]
Tu.A.372
PHYLOGENETIC ANALYSIS OF HIV-I/2 IN CAMEROON BASED ON THE POL AND
ENV REGIONS: EVIDENCE OF MIXED-INFECTION
tskesissa, urD*, Zekeng L0<, Miura T, Ida E-i,Yasnashita M, Mborlr',ka I. (;,te -' -ifG #,
Iscm i L, Ksptuc L C. Institute farViius Re ocrca, KyotM li er It, IyI, J r can:
5 entre Hospitslier Univer sita reYaounde, Careroon; #Max y'n Pttenkoer Institute,
Uni versirty ef Mcunicfh, Munich, Geanirary
Objective: o cari he olecu ar epidemiology of HIV in Camer nr, t' canssess the
incibosn o mixed-infection sod recambinatuon.
Methods: Forty eight HIV (17 HIV I, one HIV 2) were obtained fir, i onian AC,
ARC and AIDS patients in 1994 and 1995. Part of the pol regior sutabe or oparing all
the groups of HIV related lentiviruses and part of the HIV I ei uegion nIudung the V3
loop were amplified by nested PCR, sequenced, and phylogenir r, 'r nyrI

Results: The majority of Cameroonian HIV Is belonged to clade A (32), but the rest
belonged to various clades: clade B (2), clade C (2), clade D (2), clade E (I), clade F (4) and
group O (1) In the pol analysis, two different types were found in four individuals (HIV-2a
and HIV-I clade A, HIV-I group O and lade A, cla des A and C, and lades C and F), sug
gestirn double irtfcctiorns with different HIV-I subtypes or groups. Moreover, three types of
the env sequences (clade A, clade D and highly divergent group O) were found in one per
son, suggesting a triple infection. Concerning the phylogenetic topology of HIV- I based on
the poi region, group M branched into three subgroups ae and y at an ealy stage.
Subgroup or mainly consists of Af ican isolates (clades A, E and G ). Sutbgroup consists of
isolates from central Africa, Europe and United States (clades B, D and F). Subgroup y con
tains isolates fLom central Africa and India (lade C).
Conclusions: Various mixed-infection such as between different clades of HIV- I group M,
between HIIV-I and HIV-2, and even between HIV-I groups O and M were confirmed at a
rather high frequency (around 10,) in the analyzed specimens. Some of the data suggested recombination between different clades of HIV- I group M. In addition, a case of triple
infection was the first to be reported.Thus phylogenetic analyses based on the pol and env
regions provided a new insight to the understanding of HIV phylogeny.
J.Takenhisa, 53. Shogoin Kawahara-cho, Sakyo ku, Kyoto606, Japan Telephone: [phone]
Fax: 81-75 -76I-9335 email: [email protected]
Tu.A.373
PREVALENCE OF HIV-I SUBTYPES IN ARGENTINA IN DIFFERENT RISK GROUPS.
Fay, Frab., Carmpodonico M.*,Taborda,M.**, Janssens, W* **, Heyndrickx, L.***, Fay,
O.. Bios Rosario, Rosario, Argentina, *CTSR University of Rosario, Argentina,
**Institute of tropical Medicine, Belgium
Objectives: To establish the prevalence of the different HIV I subtypes in Argentina, and to
correlate it wih the risk group, time of infection, sex, age and clinical status.
Materials and methods: 45 HIV I infected patients, with known age, sex, risk group and
clinical status, were included. in this study From blood/EDTA samples, peripheral blood
m ononuclea r cells were isolated, and DNA was extracted. A 700 bp friagment from the errnv
region was amplified by a nested PCR. By means of Heteroduplex Mobility Assay (HMA) all
fragments were genetically compared with 9 previously characterized strains belonging to
subtypes A through F.
Results:
ri gr r.,,,,r,,,hltSW,' n ', N i ',.'"
3. t / (1 i 0 I,, ~, _,.
i i_) Ine t ), cm 5U5'sers -SW: S- Sex Worke s PT= Potansifusion - 5 S ymptomt c AS AS smptonmi
it 1i c I" c, ry
Conclusions: The prevalence of genotype B and F are si milar to what we have seen in a
previous study on Ar gentinean samples.The same subtypes are seen in Brazil (neighboring
couriti y), where subtype C is also present with a low prevalence. No significative differences
were seen between the prevalence of genotype B and F in relation to sex, age and clinical
status. Although the number of samples is low, genotype F strains seem to be circulating
amon IDUs, while genotype B strains are more frequent in HETs patients. More extense
studie should be done in order to confirm the rate prevalence of different HIV I subtypes
i Argenutina.
F.F. Fay. ftc Roca 740, 2000 Rosario, Argentina,Telephone: 54 I- 4 - 261234 Fax: 54 - 41 - 262913
Tu.A.374
VIRAL SUBTYPE ANALYSIS OF HIV- I INFECTED PATIENTS FROM PUNE, INDIA
fadkarLeDpakA, Moore, D., Sheppard, H, Mehendale, S*, Kulkarni, S*, Bollinger, R#.
"Nat onal AIDS Research Institute, Pune, India. rCalifornia Department of Health Services,
Berkeley CA., and #Johns Hopkins University, Baltimnore, USA
Objective: to identify HIV- I subtypes in samples collected from HIV -I seropositive and
seroconverter patients attending the STD clinics in Pune, India.
Methods: Blood samples collected from 46 HIV I-infected individuals attending two STD
clinics in Pune, India. Of these patients, 26 were seropositive at presentation and 20 were
seroconverters in a prospective study between June 1994 and July 1995. DNA was extracted from peripheral blood mononuclear cells and Heteroduplex mapping analysis (HMA)
was performed to determine nucleotide homologies with reference viral subtypes within
the V3,V4 and V5 variable regions of HIV I genome.
Results: Of the 46 samples studied, 44 (96%) were identified as subtype C. One of the
patients was found to be infected with subtype A and the other with subtype B. All subtype
C samples were further analyzed for their maximum homology to previously characterized
stubtype C reference strains C I (Malawi), C2 (Zambia) and C3 (India).Twenty nine (66%) of
the sarples were most homologous to C3 while I5 (34%) were most homologous to the
C2 reference strain. No significant difference was observed when the data were analyzed
on the Ias- of seropositve or serconverters' samples. In addition, most of the C3 samples
were closely homologous (-5% nucleotide mismatches) to each other while C2 samples
showed more divergence (10 15% mismatches) within the C2 genotype. Presence of vari
rots an ii u species was observed more in samples fLom seropositive patrents than sercoerrtenurn rrspectiye of thre C2 or C3 genrctype.
Conclusion: t ese bats reprt the lar ges anrlyssto date of HIV- I viral subtypes firom
India from 4oth seroprevalent patients and secent ser conver tsThee IIV-I subtypes (C,
A crud I) sob iwo genoetypes at C subtype 15C2 and C3) won-n identified usnsancples collectso fir ii. it s-its ate sane clinics. The higher genetc bivergence absersec n C2
ronplc, sr-ggours an erlien initirobuctin in lcdia.The C_ 3 genretype nay repsresenat a mare
recent in rt fonal variant of subtype C unique to Inda
D[).A. adkai. National AIDS Research Institute, 73,G Block,MIDCBhosari, Pune 4 I 026,
India.Tel:[phone] Fax:[phone], E- mail: rootnarernet.in

Qo
0
V
C
Or
u
c
OD
0
a
C
0
U
c
0
(-Z
c
n)
C
X
224O