Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Tu.A.281 - Tu.A.285

Tuesday, July 9, 1996

3/011 fagpo "Sp o ms nArnoip 0ogess' S.A pri 3i0Ssoli anBlinIn pi 0 V..
Lely (p<0.i 1) 14at 4 d i Iioot eoharcomenot of till aclvty 0 in IogC I CIBel v
200 I C thin 3"it '/att I 11(11ga1, p[i positivit/yCin /t0% ucaseo (3~0.0tt0t), f)ll Ir((!B
do C eeto 10')o Repiosesrrn /1(op C, vi13,rtli3 a relationship Betwee I o lr 1101'
reroi ial tIC' ton it adC I fi to1 tfilt wililihe di C I Oed.
Conclusion: ir' Its i cni 11311 t I ) ohort O(Il y shon/: ) ho tai lityo h C I
rep ne t o t O ~r.o f-iIV lantigons inro2) 3he capacity to irostimulate pi( iic0rs ors tn
boll Ir y(C-11_idrigp 911 1 on f te iio tI 0 1e1e00at 11110ced stases of tiC(-,disest. hi
indcae aI ItOa ltI Ilt inif)11 ItheC'globa l 3C1-1I 3ICI t 10181a0nst 1IV andIC11open prCpectivev
B. ARIRANC, irooii ll l I0'C hI II C, (ER 8, PA (-'IRS 82S (RiI-1Pitre Sil deti sere 83 Fad
do '[I I /l~l501 1 [ARIS ICI (5n31)4.21/ /1 8! FAX: [phone]
Tu.A.28 I
KINETICS OF HIV- I SPECIFIC CTL DURING THE CLINICAL COURSE OF HIV/-I
INFECTION: FURTHER EVIDENCE FOR A GENERAL COLLAPSE OF CELLULAR IMMUNITY
M.R.FbI l 1 r oeI,AM. Ii fbI ii' 1S R P11 Ii If Gae1, t'KF Iei o f2 Fr 101
h l fsinS ric n1Fb o fIopCI K l'.ta)3an1d C il Cll i hO t I Rtun l ' tietUniverityRof h I Cl 8 an
2L(lp 815 1111 0o y;A aenI t1Med'iillenter;,Ams terdam,1/lTh Nitielandsl~
Objective: PII'i / '01131,i( otedo-)11 10 iiti)fGag pollhIfi l oto-iI li ([lila t0 ((lIi)
(hI it t 3) 113/A 11381.13h5). 111' /O xtdtCOCo IlfindICing y iVI,tIdiialIstuie
on ihI, toi, i i Ind ti 1031 11' Indv/ l Il oadi oirr101term IOCi. 01ill in
aid pICI re,,Cl/I,1 t[ AiDS.5
Methods: IIi / 31 icCtI I I'(C ~II/1d1;i irouin1g11 lt ' 1101 1 ( h 3IiCfectod iltil
011c011binalnt Va(InI I/11 [I / 10i ( -115 I 1d01ivld g81010 Sl~d CIII) I' I II3 11 for
IlI ll i 1II iti i 1 313t VIw 3ll l '(-[if on 1 C' 1 / ''ea s y vVI usieC' to lieterln I l ll ftfI prl
curo freq18 uenc1i1es ICIV hI lllar 11111 RNI A 11 1 a/1 itl III 1 0 1i cl onC ICs laion11 V rol
OCillwe andI lnlt Iit ' [Iv A'11A IsC 311 1 11)
Study Population: I o,I stoll 1111 '1ncr 1, i IC'1C131 itiaeol' los0IrO l ll WVII
lorniidIDSift eilforIlIo11e'tIh 8II r ithl 100lLC4I ellOlit- I II 1 (1
1110) /013 113 ipa!'i' ~tIon(11111 All sr ll!. i(llot stud111' oAIDIS vode, 'lCop11
AID)S yithiI 18' 6 (II is t"l'lIlS V I (rC~I o.r 1I ("n-h).
Results:l'or, 11111 I II. 311 3 ( ii _ 1 31 p f,,iIIC /la l load w (21(,ol' i '.'e1iII 1/1
Le01'i r r 01111 b(I llr ' Ip II Ivrac l;I 101/0.01, 2a1Cof11 1110111 Ip 1113o101
sale iL I ' II 1131' I t rrr~o(;Il1(((il ul ' I9111 tI ll 11 IsecfcC.iI epinc
Conclusions: iletics, fI 1310pe ificC71 is;i(I1 401113oI pi 05211 1111r11 l i o urIeCoI
dIll t I,r t r I I. I I Iiir. 1 /110 1 1111I 't irll')11111ualld11p1 te311e1pC eSn1c3)o1i' broa1d.l
dircte aiiI dI vi o 1 1 C-I 1 I l I th I15I 1 11 0,~1tIO 111 s. It,1119 1 0 01101IV
M.R. I(i 111 I 1 11 0 1 (X A I n>di I I, eH t i d
li I 113 203,I1 1 /1 3 I 2 O ' 2 3110 III 1 13th 10 1 I/(' Ifno, 1111~n
Tu.A.282
EVOLUTION OF HISV-I REVERSE TRANSCRIPTASE SPECIFIC CYTOTOXIC T CELLS
(CTL) WITH DISEASE PROGRESSION AND ANTI-RETROVIRAL THERAPY.
Fran ir n; C 11 ii 110,111,,~ii

Tu.A.283
HLA CLASS I PEPTIDE MOTIF FREQUENCY IN HIV- I PROTEINS CORRELATES WITH
THE ALLELES ASSOCiATION WITH DISEASE PROGRESSION
Mann, h 111 1 I ', ' oedo t J'', Nelon G*. "Iromur 030001sSoction, 'Vira
Lpidenaiol;;P,.!f,1,! f r odor oh 1nd Raokille, MD; ''hOiveri ty of Alaba100
8B11mingham1/, Al
Objective: Io / C"0'tI 1' ntaimmtuarnologic11echanism01that iaght Iaontf11or h Isca
1t1ol ofiffere001I IA itlFIs with Irapid o01sdow HIV- Idisea110 p1rorsion
Methods. On hued'iii.11ihi ly ingy 111m3d'100nlwthlknow 1ew0conversio01d11110an0 CIlhow1'ed
for -> 10 yearl were typedIfor FHLA c181vI alelet addspecific alees ideoti1i0d that wore
associatdt wsith relativ/0r1t10 of disease plogressioOVird protero 00000nc05 hon 12 Gtdo
B viIue 050r ~l1181ed IanI the frequency of HLA cIasI I allee pecific motifP d0fined by
aino11 aoid anholr01poasition enumer01atedCor 1relation of thesoe ireOquencieswrthrtho 10laives
hazarftholle a lo0leV le0r0e11/1ned.
Results:l 110 00110 al 11 of peptide ototif frequency consersed irnP10 3 2 viral 0se1011Ce
ithe 9Imaoir10pro tel 5ins11Iandttavirus111a1e0shown ro tah ol0o/i03 table:

31)9
psirlions flf)t.7

31 I
0.54
001

gp120
07
0.009

31 I T1tal

Objective:V '1 1111'''1 'IIC;13c
rnii n d f(' n i I r' 1,.11I(I If'; i

Io11H1 10' r 1' sIi iII 0113111 1131i

Conclusion:'1Iheresls 1oI iteostudies identify I LA class I aleles IthIt ire asscatod wall
vdriable riles oh diIsase 311113110 100 1he in dirng that there is a c oroelai1oh o the hre0q3e1c1
of comna t Iion)Ir aino1w Ids111thaIt anchorI potentil tC TL-diirected antigenic peptides1to dif
ferin 011lass I IaleleswillhI lhe llelesd5assoian with disease progre si00provides ircto into
an immunolog103c 1 C l lilcnirnt 01 ay eaplain Vdliale ateles of disease 311ogressor inFHIV!
Ifection.
Lea I1_I Maitr, lNtationar ~I Ic ri Insititute, B8113 560/Poe 21 -78, FrederickMD 2 1/72-1320
Telepone001 33846-58 30, [AX-101-. 816 1909
Tu.A.284
STRAIN-SPECIFIC CYTOTOXIC T LYMPHOCYTE RESPONSES DIRECTED AGAINST
HUMAN IMMUNODEFICIENCY VIRUS TYPE I ENV
BollingriRoIitiI(, il 11111RF, Lubadki N, Dhi ovd B, Ray S. Johns Hpkinr University
Scoo of01 i Medic inl, Baltimre1, LMarylaiid
Rationale: Avsas o rf HIl I 011. pecific,0141at ai T lynmplocyte (C1F) 1c1ivity ypic01ly 1me01
voie respoansevsdir 001001 dgdiissI Iddar d I bor.1101 /straills hfRHIV-1, soch as 1800rMN.
Ns IcanidatItt 1111ac'rones are also based or [he same strains. hiver thet d 030le10m00
dod chrarage cai a ia, C:3tIt.iecognitionl 11111the natual avarirants of HIV- I ornv8 10a1elIe
genetio variability, 11e0specific ole oh env-specific CTL isdifficu1110 assessauing3 standard1
FHIV- I strailns 1starge01t ntigenr
Objective: Tl ompare IIOald IaraICterizthbe CL responase directed a1310s1 HIV- I eor I
ltand11rd11labor atory1 dlr5a1ns aato0301usvialstrains frow [V-Idiv nfectodO 00.uls
Methods:lhiogp31 2101codrin3gos110enewas aipopfied frmn'onnal dDNA isoited hrorm -
iadividuals with i11l.-I rrferinilthe gp 120 gene0s oere clone'd and expre'svol ir Vt~InI~
IT tiity111 a;rnt aitl 141115 env as liar ed in direct arad 00 111lted F3831t 311
011s s pecificCL 1 otO 1w0r0 is01ated aiid chariactorized.
Results: CT11 ativitylrrcteld 131i0s1 autolio31uCs5env was grelater than act., 1 d," 'e
1g1in11 IIIB ersin Ioth sLorrty. lrisostrin10specifi CTL activty was meiathr L>,'I.
iells arid 01.151)111h 1ourfiientlinI Ibien lay the ose oh artolo03us 131 0 exresig'(
ififererntil11 l Asi I ialeles1.01e0solated 11111 haractei aedhrora a"n ibjet.Is3 11 n0s00 e0
13nze aut11110o03111118, [II Iarn11R h IV I or V.Thiee clones werorain'10pec 3,inc1d101
clone11hat only rI,itzdraut tologoos andI MN env.The maseofh odo n a cInsIracts0efrsin03
111110cIlled FIV- I 011 / lizedIte 3TLeprtop1110fohItese cones to 3 dIllfe1e1t10e9i0n 1 ofnv
indniltCrOII 111tion ofthemlirem111 l/epaitopes oh these CTL cl0001 It 00g3ing3
Conclusions: 51oei I Iinhoc tod idivi doals have stin-especific CITF cii Vty Stnard 11
1s1)s dioInat lull detect o 1 ii actorr ie 0115 specific CTL actrvity An effective CTL 10sp01n50
Oa 1reiquire i Lroaad, p a yo Ord Iresponse diirected at coinser ved aiad varoiab(train-10e00fic)
e'pitopes Chatractorizatall of stirtin-specifichCTL. activity may have impor tant wplit infar
tutdelrstandling1HIV/ path'ogernevisand vacciOOe1d01130.
P.C Bollingr;ohnsrHopkras(Iniveirsity RossResetoch B81l1103, Room1 159,h20Rotlarnd
AvenuoeBaliloaeIC1Il) 212(5, Lel 410-6 14 0938 Faa. 410-955-7889,
Fimail: Rhl.BnOC'el I raf we ohh I.od
Tu.A.285
SELECTION OF VIRAL EPITOPES BY CYTOLYTIC T CELLS FROM
HLA-A I I HIV INFECTED INDIVIDUALS,
Cil0annl-POOC 010131 Beatric'.hotihet J-A, hlomaicl F. INSERM U44,IC l,h1M75014 PanrsFra1100
Objectives: To dotermn te how HIV infected inidividuals select their CTL epitopes / 00 1103
to their-lit A phenol~type.
Methods: Tinr00 III / positive' FI A All individuahls were tosted forItheirr lyt I I.tv141ga0n1
10111 FIV epitopes oslicted F1yFIFA-All.TLwo epitopes are denived fronm 110 NElprotoon
(aa/I -82, 84-92) and tho two othors are deived from tho reverse 11105 riptase proterir
(ai3lS 31 508-525). PBINlC were stimlatld wth 1ltt00lI' irra11ted PFHA 13blltad
coitinied holrI14 day'.. hroaium 1111Ieledased5ass9ay perhonrmod to evalulate CAL ac1tvity
Results: We fhrst shor'e I that tihese thir00 siuijects are 11ifferent conc010i03 thre rechoniia'
oh loesedifret-iot lA-Allirestriclod epitopev.VWo demonstrate tnat one o hem en 0o
arzed oray the po0 325-333 peptide svhereas the secorad o000r000g0ied both poipep;'d1s
ted the tird recograizedIboth Ilef pept dev Th eve rsilts radicate othor a pheromenoCr )f
irtinto 11 1111 0100oothe oxisteorceoo sctping niotanitswhichlavoi11 CTFL 0ecgn iion
Fxperi1e1001oh diluonoohthe peptideo Juing 03eII o 11031 1s1)9 Ievedled 11fhere00e0
betweenat110101epitopes. The ctatacty ofha g3.10peptide tIsenitoetargets 1o 1 11a
very low cancorn Iiololl0ev 001 0001 10 10 coIrelated with rhocpipty of h is pept 00 1t
be rc'oIniCzred by I alidividuals. Analysis of autologous HIVse1q11ene odr-wed 1fro01 Ih0eCe
subect w01Aill illowIA' oinv0 st0.0.11 a I o ib Leesc011 gto 31hbC ytol111I 0re31oIIe0

Methods r.l bia, f1IVp(._I I It13' 1v Isl. pa o- i ioyCr srr
a1 coh rt -;f5(). 1 _ I ll( I )} nil I0 'r0 'a 31Cre r nrlatior ingl alit 01ou
iriIib, lIIA lA.r c'ip _.,I _p,,,rI. 281rto' Po'lalvemo'-estd on.so 0.1II bt ri I'i l I I,ndSyI i mrc olrril I I epide.'bill0Cai s
sehira 01. o 31 ll '1 I..'(WAYbIC lb C'11, iIC' In'sted Ii 14
Resuilts: C flIqv f1r1WlI/t Ilib.l 3p1otils Pat, as'toill tvsr.Er itGraloll' is luric
str FillS IAl 'r 1111 e~alein0',x; ofthe'patients.t1 stir soflbJrhr lob rThe
N11123halfoflthe 'ii f i ' ~giid bI7196rrandlrhhIOl harrlfhby54~%ohthe' atients
tetld.1Irt uro'tirr.'l, bob' 1 1111 lytitOa lCr disas,whn/l'i1041 Iuntsv In' bst
2(333m iniior i' pitsl, 1111at'eceived Iirrroiril Ithcoapy (I I 3atrints), h. It.we athe
diectedrtotile conIervId hhhhlhl otrmo llthin tathre re ',iriile NI12iialf
(N CIIr3n r:II tv Puss l.-ers/A 131110011tV)/Irhrastrudying9 2 progressors oer.ithiree
years tine it od, '/1' Fosooled a hltoh Ri Icogit1110 onam th3dNter'lminal epaitopas ira
re13o Ia 310 3 I l/Ci 11. Corternone01sIC inbein6/It-It10.iHowrever, uling; peaptide
'p1' if loet r ii I.11 IL piicursoIslbspec11101 foirrp3101359-181 coild stril l' ex10nJod it
vir.I I II lv1 i a LIF-It peidinterIlactIinin piit111 31c'.e1001111 310 46 354,
38-316. 'Iv iiias opotr4/6 181, 588-59e 615-653. 581-691, 695-707h tand 69i-7'18.
A0eI(e IhiracIite r z.1 II C'v1sti 1ate Aw l'tlirb C IIvir l irt thor Iith at.idu101'rsistan t o 1 a11ti1111 01 I I ohd1/5sIlly1/ause CF Ineficriency10, thoe1impadct f scli muttionrs (namely I P
184I.1110 RI. IS)1o1n30111 t irecgiion wllAl tuiedoi. In somre lpitapes l lA Ibinding aII
C 13 I rcorleaIeeiedato ivrbrogated by1 1 r Ii i l ititoly, v/11110 frirors011 ec'ilfail r
eI,; _ AI I t I re 0 lob Ii I
Conclusions:.111 1.11 1651 1 11111at t I t r,o rutrrl iof Ill. I R I s rairr liit I '/nh diieaso
311 1' 1 1.' 11111' lu sihtsiii nd rn rrii c', lilt rexpansrion ohhC IL specii ohl tir11
rnant mit ti iii)miillIi i o rri itio iii I r'I 131 bily avo1 idI'theiimerince0of'rag13rsi'
til r' II thus / 1par i.' pc C ii 1' forIillill lb CIII iiopy.
( ItrI it or loerr ' Iirrrr a toicC~lir ii ml I i 1110'C114/,([IPRSiIRAea1,
Fax: (t33 1 2 1It /111101,reba i ml 11 fr.

223