Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Mo.C.1665 - Mo.C.1670

Monday, July 8, 1996

Mo.C.1665
HIV-2 INFECTION IN ADULT PATIENTS:THE FRENCH NATIONAL COHORT
M ither on, Sophifr*, Puyo S' ", Lepri 'tre A', Sirnon F -, Chine G**, Salamon R***,
Coulaud J1*, Bru-Vzinet. Depts. of *Inlfectious and Tropical Diseases and *Virology
lH-pital Bichit Claude Bernard, Paris; *INSFRFPH U330, Bordeaux, France.
Background: A rulticentir nihional cohort study as been initiated in May 1994*.
Objectives: to des>cin ithe flh atra t1history of HIIV 2 infected adult patients (pts); to
establish clinico- virologic, iorrelations for progression of the disease.
Design: I) Inclusion cr iteria: confir reed serological diagnosis of HIV-2 infection alone;
age " 18 years:; informed consent: potertial regular and long follow-up. 2) HIV-2 infected pts
not rreeting tese tea Stria and p with IV I Iand 2 serological profile are only listed.
3) Follow up: in addition to inclusion staitus, epideriological, clinical and immunological data
are colc F ti' I evi rv C a I it); (rr )i I rtvirolo icl samples (serology, peripheral blood
lymphocytes rnd pai I rina) once_,yea >,, d in cae r of intermediate event. Eight pts meeting
this design ivep ben r ct io;'ect Iel> iciued.
Results: By Noverberr I)93, prelii rry sur vey estimated the number of HIV-2 infected
pts living and olIlowed in Franre to be 1)2 to 132. By January 1996, 98 pts have been listed
and 72 pts are enrolled: 30 I ert) 12 women, mean age: 39 years, originating from Europe
(1 7),West Af rica (5 I),West Indies (3) arid torocco (I).The mean duration of French residency is 121 rutsin the 58 pts not born i scontinental France. HIV-2 infection is related to
heter oscxualc rntacti (n C0) precious transfusio (n5), homo/bisexual contacts (n=2)
and unkrown in I cer.Fli' ie resric ed site of c ntarmiration is West Africa in 47%, France
in 28~ of cases, ert one el atSI other in 22%, and riot determined in 3%.A previous connection with VVest Africa is orruid i 75?, of cases.
At inclusion, 53 pts were C(tDC group A; 8 group B and I I group C.The mean CD4 and
CD8 lymphocyte count (/r t) (and perentage) were respectively 465 (28%) and 672
(45%). (:D, lyrrphocyte count in AIDS its was 162/mrr3 (I 5.5%).Thirty two percent of
pts were receirving iiS tirovirl therapy, rid 26% penumocystosis arad toxoplasmosis prophylaxis. By Jainuary 1996, lthe mean duration of follow up was 17 mths (I-77); one death
had bee reported,,Ind ei pt wi s lv Iot to follow-up.
Conclusion: theereliminare i ro 'ults provide cross- sectional view of the characteristics
of more than half the' cases oft IV 2 rinfecid adult pts known and followed in France.
S. Matheron. Hopital BiBIhat Claiude Bern rd, 46 rue Itenti Huchard, 75018 Paris, France.
l l.: (33-I) 40.2 R.7f383; Fr 3 3 - ) 1).9.53.00
Mo.C.1666
HIV-2 INFECTION IN STD PATIENTS IN PUNE, INDIA.
Mehendale Sacy, Risbud A C, angakhedkar R, Divekar A*,Thilakavathi S*, Paranjape R*,
Gadkari D*, Bollinger R", Rodht igues J '. ' N tional AID)S Research Institute, Pune, India,
"Johns okins i UniversJity, Baltimo re, ISA
Objective: 1a deterr rine the preva lernce of HI1V2 infection and study its relationship with
sorne biological and behavioral risk fa tors.
Methods: In the IIVNFI project in Punre, India, 5668 persons (STD patients and female
sex wor kers) atternding the two SD clinics were evaluated for their prevalent and past
STDs arid screened for -HIV infection between M"lay 1993 and December 1995. Information
on demograptics, sexual anid rmedical history and AIDS wareness was collected.
Results: (')ver ll prvalence of I lIV infeclion was found to be 20.8% (1180/5668); much
higher anong women i (27.8i) thar in rne' (I 8.4%). HIV- I accounted for 95% (I I123/I 180)
infectiorns. I IV2 for 1.7 (2 /I 1180) and 3.3% (38/ 1180) were dually reactive.The prevalence of tlV2 ifrtion was 0%(3 1" (21/5668) and the period prevalence remained constant
throughout the study. Female gender was associated with higher HIV-2 prevalence with a
borderin e significance (8/238 wormen Vs I 3/906 men infected either with HIV I or HIV2:Chi sq 2.89 p 009). (:ornrer cial sex work was related to higher HIV-2 prevalence
(8/204 CSWs Vs 13/9,10 n C( SVWs infelcted either with HIV I or HIV 2:Chi sq 7.4,
p 0.03). lajor ity lt f re per sos infected with IIV-2 were above 20 years of age, Hindu by
religion, unrn, ied iriel cr o ates tad only primary education. No significant difference was
obser vedI rainy te peros ifectSd wit I IV I and HIV-2 wirth respect to these factors
and other t ctrs like ondom uset, tattoori' since 1986, history of multiple medical injections arrd STDs n the past. Vost (62%) had no previous knowledge of AIDS. None of
therse 21 had eithe.istayed in.r had a sexual encounIter in Bombay city from where the
FIIV 2 infect io ItIas beeFn repot ted previously. Chancroid, syphilis, candidiasis, warts and trichomoniasis we cthe io monly obseved S 1 Ds among the per sons infected with HIV-2.
Conclusions:AItfiouIC repor ted primar ily f ror Western Afi-rca, HIV-2 infection has also
been docurented icI c ties like BoIay irid Pure in Western India. HIV-I was commonly
prevalent,,irin. the S 113 linic itterdee, in Pune, however; few infections with HIV-2 were
also osCr Vr'd. Ihe relationh i bartveer ictors like condom use, sexual behavior, STDs and
their tretSrient and r her i I IV2 rfectonm rust be carefully investigated.
DrSanlay "lehendale,/ssistant.,rctoir ca ltional AIDS Research Institute, 73, G Block,
MIDC, Bhosari, Purr II 02i16. India ll:212-791 312 Fax:[phone], E-mail:
root(lcnir r eti i
Mo.C.1667
GEOGRAPHIC CLUSTERING BETWEEN SIVSM AND HIV-2 IN WEST AFRICA AND
IDENTIFICATION OF A NEW HIV-2 SUBTYPE IN SIERRA LEONE
ClaenZhiweiI,Teler P, Reed P, Zhang L, ettie A1, Luckay A, Sodora DLI, Ho DD,
Marx PA. Aa ron Di.mor d AI)S Resarch CeFrter ard Department of Microbiology,
lew York Unver sity "edical t er; rtaw tr itk, NY 10016
Objective: TIs reserc waS to study the phylogenetic relationship between simian immunodeficiency viruses (SIVs) thrat ie na turally I arbored by sooty mangabeys (SM, Cercocebus
torquatus atys) in West Afric i,ind hunar imrunodeficiency virus type 2 (HIV-2) in the
sarae Ie iona at VVe, S Afr C i.
Methods: We geiretically h urcter zcd sultrple sn'w SIVsm vr uses identified from 3 of 4
rdcult (crl rd 13 of5In 9i truetld~ il soty ru~n giaeys, rod HIV 2 strains from two persors in Sierra tone 'r nd r iberia, Pr lvrr al cv and env gene fragments were amplified fl-om
urncultuired Iioat relIs anti> inn I' I -'Cli id s esencrd.
Results: Br cd on ghyretc i nly s at gce fragrents, all newly characterized SIV and
HIV2 strains were riermbers of the SIVsrr/HIV2 farily but were highly divergent from one
another even w ithin a sinr, fer al iroop Irrportantly the degree of genetic diversity

between new S!Vs was equivalent to that between HIV-2 subtypes.These results. together
with the high seroprevalence in adult free-living feral SMs. strongly suggest that SIVsm has
existed in West Africa long before the AIDS epidemic. Strikingly, 3 of 3 SIVsm obtained
from household pet nmaungabeys clustered with HIV-2PA (subtype E) and HIV2F0784 (subtype D) which are sub-types found in Sierra Leone and Liberia respectively These data
show geographic clustering even at the village level, between SIVsm and HIV-2 viruses. In
addition, 2 HIV 2 and 7 HIV- I viruses were found in Sierra Leone from a survey of 9,309
West Africans living in rural areas.This indicates the zoonotic transmission is raure in rural
areas where positive Sms occur Genetically one HIV 2 from Sierra Leone was highly divergent from all known HIV 2 subtypes and SIVsm, indicating a new subtype F in defined geographic areas.
Conclusion:The data provided new and broad support for the hypothesis that each HIV-2
subtype in West Africans originated from widely divergent SIVsm strains, transmitted by
independent cross-species events
Z Chen, 455 First Avenue, 7th Floor, New York, NY 100 16 Telephone: [phone] I
Fax: [phone] email:[email protected]
Mo.C.1668
STUDY OF HIV-I/HIV-2 DUAL INFECTION IN BOMBAY
Zhang. ling!, ManiarJ Ke", Saple D G"*,Tsuchie H", Kageyama S., Wakamiya N*, Kurimura
T. *University of Osaka, Suita, Osaka, Japan; 'G.THospital, Bombay, India
Objective:To estimate the prevalence of HIV-I and HIV 2 i n Bombay and nvestgate the
interaction of HIV-I and HIV-2 with respect to viral replication in HIV I/HIV-2 dually
infected patients.
Methods:Blood samples were collected from 60 outpatients in G. THospital, Bombay
Seroprevalence of HIV-I and HIV-2 were investigated by the particle agglutination (PA)
method and indirect immunofluorescence assay (IFA). HIV-I and HIV-2 proviral DNA in primary peripheral blood mononuclear cells (PBMC) were detected by nested polymerase
chain reaction (PCR). Quantitative competitive polymerase chain reaction (QC-PCR)
method was used to quantify virion associ ted HIV-I and HIV-2 RNA ins plasma.
Results: According to the results of PA, IFA and PCR, 60 Bombay patients were classified
into five categories: 32 were HIV-I single infection (53.5%), 15 were HIV I/HIV2 dual infection (25%), 3 were HIV-2 single infection (5%). 7 were HIV nonirnfection (I I 7%) and 3
were indeterminate (5%). Although the mean HIV I viral load of HIV-I/HIV-2 dually infected group (79,330 copies/mi plasma) did not significantly different from that of HIV- I single
infected group (86,640 copies/ml plasma). but we noted the extremely separated distribution in HIV-I/HIV 2 dually infected group (<6,000 copies/ml plasma in 6 patients and
> 160,000 copies/ml plasma in 4 patients), comnpared with distribution of HIV I viral load in
HIV- I singly infected group. Despite the viral load of HIV 2 lower than that of HIV-I in the
same HIV- I/HIV-2 dually infected patients, we did not observe the significant difference of
HIV-2 viral load between the HIV- I/HIV2 dually infected group and HIV-2 single infected
group.(This comparison may lack statistical power due to small number of HIV-2 single
infected patients.)
Conclusion: In Bombay HIV- I infection is predominant and HIV /HIV-2 dual infection is
more common than HIV-2 single infection. In some dually infected patients, the existence of
HIV 2 may have some inhibitory effect, on HIV I replication, but the order of HIV infection
and subtype of HIV-2 may be crucial: whether HIV I can not rephcate well in previously
HIV 2 infected patients or whether only some subtype of HIV-2 inh ibits HIV I replication
require further study
J. Zhang, Osaka University 3 I Yamnadaoka, Suita. Japan Telephone: [phone]
Fax [phone]
Mo.C.1669
HIV-2 IN SPAIN
Gutierrez M, Soriano V, Caballero E, Cil a G, Dronda F. Gonzalez-Lahoz J and The HIV2
Spanish Study Group. Coordination: CIC, Institut de S laud Car los III, Madrid, Spain.
Background: HIV-2 infection is endemic in West Africa, where it is responsible for many
cases of AIDS.There have been described HIV-2 infected subjects in other countries, mainly
in African immigrants, although also in natives. In 1 988 there were identified the first cases
of HIV-2 infection in Spai.The HIV 2 Sparn ish Study Group was created in 1 990 and it has
collected information of l sew raesof HIV-2 infection identified in Spain.
Results: Up to January 1996, there have bees diagnosed 57 HIV-2 inf ected individuals, by
the majority around large urban areas (23 cases in Madrid and 19 in Barcelona). All were
African immigrants, except for 2 natives (2 %), of whom 7 had acquired the inftection uin
endemic areas; the other five (two women admitting sexual promiscuity and four
homo/bisexual men) most likely had acquired the infection in Spain. Heterosexual transmission was probable in all cases, except for n 7 subects: five homo/bisexual males, a subject
who likely acquired infection through parenteral exposure, and a child born from a HIV-2
infected mother. Nine patients (all born in Spa in) have developed AIDS and 5 of them have
died.The incidence of new cases reported annually has remained estable since the beginning, regardless the increasinVg nmber of case found rt Spansh bon subects.
Conclusion: HIV-2 infectri us present uins Spar. a3 twere us evidence at errergpig ongoing
transmission outsidhee group of African im mgrnts. However, Iee s nocn rapid spreading
of a HIV-2 epidemy.
Dra. M. Gutrerrez. CIC, Instituta de Salad C i all.I C/ Siset a Delg iaI 0. 28029 Madrid,
Spain. Phone (34-I)3 14 0807. Fax 733 66i 4.
Mo.C. 1670
COMPARATIVE STUDY OF HTLV I/Il SEROPREVALENCE BETWEEN TWVO PERIODS
OF TIME IN BUENOS AIRES. ARGENTINA.
Vazquez Anub:al 0, Deluchi J, Cafirza A ",Cattro a K, Benetucc rJ '. Laboralarmo de
Betrovirus y virus Asocrados FUNDAI, Sala 17. HaspiSal Mariz, Buenos Aires Argentira.
Objective: Comparison of HTLV i/il seraprecalerce between twa graups.
Methods: 2815 serum sampret obtained traom twa graupt an tree testng patients seeking
for HIV, (A) since tune to september 1994 and (B) sn the sme perad of 1995. were tested
for HTLV I/Il antibodies by PA (Seradia Eu1iebaot arO ELISA (HTLV Organon Tekr ka( as
screening. Reactuves samples were cantirmced by lEA (in hau'e). lEA reictives were tested by

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