Abstracts Vol. 1 [International Conference on AIDS (11th: 1996: Vancouver, Canada)]

Mo.C.1447 - Mo.C.1453

Monday, July 8, 1996

Mo.C. 1447
FREQUENCY OF SUBTYPES OF HIV-I IN PARTICIPANTS OF A COHORT STUDY IN
RIO DE JANEIRO, BRAZILAS SUGGESTED BY ENDONUCLEASE DIGESTION
PATTERNS OF THE PROTEASE GENE.
Monica E.IPinto, Amlcar frTnuri', Mauro Schechter Hospital Universitiri. r nentino
Fraga Filho, Departamento de (Genetica, Universidade Federal do Rio de Janeiro
Objective: To investigate the presence of differen t HIV I ubtypes ina hrt'1-VI
nfected siubjerts in Rio de Janeiro, Brazil.
Methods: IBlood was collected from sequential individuals participating in a prospective HIV
-ohort study who had blood drawn for routine examination between October December
1994. the viral protease gene was amplified using a nested PCR.The amplified samples
were first digested with Alul and than with Hinf or Bcll, depending on the pattern resulting
from the first cfgestion.
Results: Of the 94 samples analyzed, 76 (80,9%) showed a major fragment of 147 or 2I1
bp, when digested with Alul; of these, 67 (71,.3%) had a single restriction site to Hinf, and 9
(9,6%) had no restriction sites to Hinf, suggestive of subtypes B and D, respectively. Fight
(8,5%) samples had a major fragment of 247 bp when digested with Aluh these were then
digested with Boll: 2 samples showed restriction sites, whereas 6 (6,496) had no Boll sites,
suggestive of subtypes C and F, respectively.
Discussion: The use of restriction endonucleases allows the segregation of HIV-I protease
genes into distinct patterns of digestion, which in turn are characteristic of each of the
known subtypes. This is i relatively simple approach that permits the expeditious analysis of a
large nursber of samples, hence allowing the performance of population based studies. Our
results indicate that it least 4 different HIV- I subtypes may be circulating in Rio de Janeiro.
This study was partially supported by a grant from Petrobris SA.
Monica E. Pinto, Rua Cardoso Junior 315, Laranjeiras, Rio de Janeiro, RJ, Brazil, 22251 000.
Tel: [phone] ff1 4, Fax: 55(2 I) 590 I 6 15, email: [email protected]
Mo.C. 1448
DIFFERENCES IN THE PREVALENCE OF HIV-I SUBTYPES IN MALE AND FEMALE
PARTICIPANTS OF A COHORT STUDY IN RIO DE JANEIRO, BRAZIL.
Monica E. Pinto, AmirlcarTinuri ', Mauro Schechter. Hospital Universitfrio Ciernentino
Fraga Filho, )epartamento de Gendtics, Universidade Federal do Rio de ]Janeiro.
Objective: Fo investigate the prevalence of different HIV- I subtypes in male and female participants of a cohort study in Rio de Janeiro.
Methods: Blood was collected from sequential individuals participating in a prospective HIV
cohort study who had blood drawn for routine examination between OctoberDecember!994.T he viral protease gene was amplified using a nested PCR.The amplified samples
were first digested with Alul and then with Dinf or Bcll, depending on the pattern resulting
from the first digestion. PCR amplified HIV- I protease genes were classified into 4 different
subtypes (B, C, D, or F) according to endonuclease digestion patterns. Epi Info version 6.01
(Stone Mountain, GA) was used for data analysis.
Results: Of 94 patients.studied, 59 were male and (5 female. No significant correlations
were found between endonuclease digestion patterns and route of infection (sexual or parenter, l) or between route of infection ind gender.Twenty seven individuals, I5 women and
I 2 men, had endonuclease digestion patterns suggestive of subtypes other then B (C, D or
F), while 67 individuals, 20 women and 47 men, had a protease digestion pattern characterstic of subtype B.Thus, non-B subtypes were significantly more common in female then in
male patients (p < 0,05). Of the 27 non-B samples, 6 showed an endonuclease digestion
pattern suggestive of subtype F. Of the 6 samples with subtype F digestion pattern. I nas
from a male patient and 5 were from females (p<0,05).When subtype F samples were
excluded from the analysis, no statistically significant differences were found.
Conclusions: The F subtype was found to be significantly more common in women than in
men, indicating in apparent segregation of HIV- I subtypes circulating in Rio de Janeiro,
according to the gender of the patient.The clinical and epidemiologic significance of the
encountered segregation of HIV I subtypes, which is reminiscent of what has been
descrnhed in Thailand, remains to be determined.
This study was partially funded by a grant from Petrobris SA.
Amlcar Taniui, Rua Cardoso Junior: 315, Laranjeiras, Rio de Janeiro, RJ, Brazil, 22251 000.
Tel: [phone], Fax: 55(2 I) 590- I 6 15, email: Maruro [email protected]
Mo.C. 1449
SEROLOGICAL MARKERS FOR HEPATITIS B(HBV) AND C (HCV) IN PATIENTS
INFECTED WITH HIV- I
Goncales r Fenando L PzPavan,MHP*,Aoki,f H i, Goncales,NS Infectious Diseases
Depts.State niversity of Campinas, Sio Paulo, Brazil: Hematology and Hemiother:ipy
Center State University of Campinas,,o rPalo, Brazil
Objectives: To determine the prevalence of HBV and HCV in patients infected with HIV I.
Methods: We studied 232 patients infected with HIV-I in the Infectious Diseases Service of
the fHospital das Chinicas, State University of Carnpnas (UNICAMP), S.Pst, Brazil, betweer
1992 1995.
Results: In the population studied, I16 (69,4%) patients were male and 71 (30,6%) were
female; 202(87,1%) were white and 30(12,9%) were black.The mean age was 30.88 years
(I 7 to 63). In initial evaluation II 5(49,6%) patients were classified in group II, 70(30,2%) in
group III and 47(20,2%) in group IV. The transmission of the HIV was sexual in I I 6(50%) of
the cases; by blood in 74(31,9%); by sex or blood in 38(1 6,4%) and indeterminate in
04(1,7%) patients. It wasnt possible to realize all serologic evaluation on all patients.
The results ol the markers for HBV and HCV are presented in Table I.

TABLE I - MARKERS FOR HBV AND HCV IN SUBJECTS INFECTED WITH HIV- I
MARKER TOTAL TESTED POSITIVE (%)
rnti-HCV 221 119 (538)
anti-HBc 226 100 (44,2)
anti-HBs on1, 26I.
HBsAg only 226 O1 (0,4
Conclusion: 116 (5 1,3%)t patients had at least one marker for HBV nd i f (1 / ),ere
positive for HCV showing the high pralence f these is ieth, o, H... I f0
subjects anti-HBc postv,' we fourid 1 1%) Hs, g positiv
and 25 (25%) with only anti-HBc positive.
F. L. Gongales jr. Av. Dr Romeu Tortima, 725. Cidade Universitcria Campinis, Brazl. CEP
13084-000. Fax [phone]-Phone: [phone]
Mo.C. 1450
HIGH INCIDENCE OF HTLV-I/II IN HIV-I POSITIVE INDIVIDUALS IN BRAZIL
Goncales, Neiva S.L, Pavan, MHP*, Aoki, FH**. Goncales Jr, FL-**. Hematclogy and
Herotherapy Center: C" Infectious Diseases Depts. State University of Campinas, Sao
Paulo, Brazil
Objective: To determine the seroprevalence of retroviruses HTLVI/II among HIV-I infected
individuals in different risk groups.
Methods: One hundred and eighty persons positive for HIV-I were assessed for HTIV-I/II.
Seventy-five were classified into sexual risk group (Group I), 46 were intravenoiis drug
users (Group II) and 59 were classified into both risk groups, sexual nd intraveno s drug
users (Group III). Serum samples were collected during follow-up for Sexually Transmnitted
Diseases at the Hospital of State University of Campinas, Brazil. Samples were assayed for
intibodies to HTLV I/11I with an Enzyme Immrunoassay (EIA.ABBOTT Laboratories, North
Chicago. IL).We considered positive only the samples which were reactive at least twice.
Results: Eighteen samples (24%) were positive for HTLV-I/II in 75 individuals of Group I,
13 samples (28%) were positive in 46 individuals of Group II and 24 samples (40,7%) were
reactive in 59 individuals who belonged to Group III.The statical analysis showed an adjust
ed odds ratio of 2,17 f( - 0,05) for Groups Ill.The ovenrall inr ider(,- f1 TI V-i/II s
30,5% considering ll groups.
Conclusion: We observed a high incidence of coinfected HTLV-I/I and HIV seropositive
individuals. Further study will be carried out to evaluate if coinfection is a poor prognostic
factor in HIV- I infected persons.
N. S. L. Goncales, Av. Dr: Romeu Tortima, 725. Cidade Universitaria, Campinas, Brzil. CEP
13084-000. FAX [phone] I Phone: [phone]
Mo.C.1452
PREVALENCE OF STD AND HIV/AIDS INFECTION IN ADOLESCENTS THAT
ATTENDED SERVICE DEPARTMENT OF SEXUALLY TRANSMITTED DISEASES
DERMATOLOGICAL AND SKIN SURGERY INSTITUTE SANTO DOMINGO,
DOMINICAN REPUBLIC PERIOD JANUARY 1992-SEPTEMBER 1994
Alcantara, Rafael, 5Suazo, M., *Henriquez,Ana M. *FerrarisAmbar*Dpto. Ets. Instituto
Dermatologico "Facultad De Salud, InstitutoTecnologico De Sto. Dgo., Republica Dominicana
Objective: Determine the relevance of the STD including HIV/AIDS infection in young
patients between I -19 years of age that attended service at the STD Department of the
Dermatological Institute in Santo Domingo, during the period between January 1992 and
September i 994. And analyze the different variables the influenced in them.
Methodology: It is a study of retrospective analysis in which I, 121I cases were examined
-orresponding to young men and women between I I- 19 years old that attended service
because of suspecting of having a STD during the period of time previously determined.
Results: An early start to active sexual life is proven in young people of both sexes. finding
- relevant frequence in promiscuous homo and bisexual practices. And last, a relevant incidence of HIV/AIDS infection in young people with a STD.
Conclusions: Proven the early start in active sexual life, the promiscuous sexual practices
and the high incidence of STD in adolescents, we believe it s important to initiate prevention campaigns for STD and the sexual transmission of the HIV in this social group, even
more given the proven fict that most of the cases of AIDSr inn c i,,ontry are estimated
were infected by promiscuous sexual practices during the adolescence.
Dr Rafael Alcantara C. Dpto. Ets. Instituto Dermatologico P Q. Box 1090
Fax I-[phone] Sto. Dgo.. Republica Domninic.irna
Mo.C. 1453
HIV INFECTION IN A PSYCHIATRIC HOSPITAL IN MEXICO 1988-1995
Magis C, Casneda Carlos, Cisneros L.*, Santarriaga M. *, Loo E., Martinez P. " del
Rio C*. *National AIDS Council (CONASIDA), Mexico **Fray Bernardino Alvarez
Psychiatric Hospital, Ministry of Health. Mexico.
Objective:To review the HIV/AIDS seroprevalence at the major psychiatnric hospital in Mexico.
Methods: Patients admitted between 1992- 1995 as well as 1988 seroorevalence survey
were analyzed. Demographic dita, seroDrvalence stitus, socinesonom it.s arid psychiitric diagnosis were studied.
Results: In the!988 seroprevalence study no patients were found to be HIV positive out of
800 tested. From I992 to 1995 HIV testing was pernormed on i,-,i~,,,s on al new
admissions. In this fhar year period 46 patients aut of 12,040 test perfor md.,, HIV positive (seroprevalence=0.4%). Analysis of seroprevalence trends by year restre, that in
1992 seroprevalence was 0.4%, in 1993 0.4%, in 199t 0.5% and in 995 ie%. Aron the 46
HIV positive patients 32(70%) were asymptomatic and the rest had.IS Mnst i/46
patients were male with a mean age 32.8 years.The most commrn po,. a v r--i,giasi
imong these infected were mental disorders due to iireanic lesions or oarmid ifisease
(WHO-t01DC: FO6).There was no statistically significant difference wt th s dh[.nosis
between those infected and not infected. However mental disorders,,, to *h, sse o drugs
(WHO-I0IDC:FI 9) ocurred in 23.9% of the HIV+ patients and were,rIr!y a~- rciated with
infection (OR- 6.41-95% CI 3.05- 13.17). Also. depression (VHO IOID C 12) present ira
1 5.2% of patients, showed an association with HIV+ (OR - 19.47 95%C - 7 77-4.6
Conclusions: At the Fray Bernardino hospital, a third level psychiatric we have not seen an
increasing HIV seroprevalence imong it's patients.The findings that drug associated disor
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