Retroviruses of Human A.I.D.S. and Related Animal Diseases

associated with growth of human T cells,
a suppressive response of the SCID
recipient, or a general shortage of human macrophage function.
HIV infection of hu-PBL-SCID
mice
A major incentive in the development
of the hu-PBL-SCID mouse was to
provide a small animal model for AIDS
research. We have now completed studies [5] showing that hu-PBL-SCID mice
can be infected either with cell-free human immunodeficiency virus (HIV) or
with HIV-infected syngeneic T lymphoblasts. Virus can be cultured from the
spleen, peripheral blood, and lymph

nodes of infected mice, and in situ hybridization confirms infection of human
cells in the SCID spleen. In addition,
HIV-specific sequences can be amplified
from hu-PBL-SCID spleen by the polymerase chain reaction.
Figure 3 shows the results of an experiment in which hu-PBL-SCID mice
were infected with either cell-free or
cell-associated LAV-1/Bru strain HIV-1.
Sixteen identical hu-PBL-SCID mice
were constructed by the injection intraperitoneally of 40 x 106 PBL from a
single EBV-seronegative donor. Two
weeks later, the mice were infected either
by the injection of T lymphoblasts derived from the same donor and infected in
vitro with LAV-1, or by the injection of
cell-free supernate from HIV-1 in CEM

0 T-- - 40 x 1 0 PBL from EBV-seronegative donor

LJ
Cr)
LL..
0
0
1,
z
CQI-J
aLUJ
LLJ
ILU

6
- 5x 10 LAV-1
Infected syngenelc T
cell blasts

105 TCID50LAV-1
cell-free supernate

I I

810--
104 -
I

0 1 2 1 2
NUMBER OF MICE WITH POSITIVE VIRUS ISOLATION WITH
Fig. 3 INFECTED CELLS N OR CELL-FREE VIRUS

176